Catalog Number | Product Size | Concentration | Price |
---|---|---|---|
GE0002S | 70 pmol | 1 μM | Online Inquiry |
GE0002X | 2,000 pmol | 100 μM | Online Inquiry |
Product Information | |
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Description | CD® Cas12a is expressed as a N-terminal 6×His-tagged fusion in E. coli. It is a programmable DNA endonuclease guided by a sgRNA. A gRNA complementary and a 5' TTTV protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence are required for targeting to the target site. This enzyme is active from 16 to 48°C. |
Applications | Random Mutagenesis |
Properties | |
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Storage Temperature | -20°C |
Storage Conditions | • 500 mM NaCl • 20 mM sodium acetate • 0.1 mM EDTA • 0.1 mM TCEP • 50% Glycerol • pH 6.0 at 25°C |
Shipping Conditions | Blue ice. |
Heat Activation | 65°C for 10 min |
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