Synthetic Biology
CD<sup>®</sup> Cas12a

CD® Cas12a

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CD® Cas12a

Catalog Number Product Size Concentration Price
GE0002S 70 pmol 1 μM Online Inquiry
GE0002X 2,000 pmol 100 μM Online Inquiry
Product Information
Description CD® Cas12a is expressed as a N-terminal 6×His-tagged fusion in E. coli. It is a programmable DNA endonuclease guided by a sgRNA. A gRNA complementary and a 5' TTTV protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence are required for targeting to the target site. This enzyme is active from 16 to 48°C.
Applications Random Mutagenesis
Properties
Storage Temperature -20°C
Storage Conditions • 500 mM NaCl
• 20 mM sodium acetate
• 0.1 mM EDTA
• 0.1 mM TCEP
• 50% Glycerol
• pH 6.0 at 25°C
Shipping Conditions Blue ice.
Heat Activation 65°C for 10 min

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