Yeast Gene Regulation Services

Yeast CRISPRi Gene Repression Service

Programmable dCas9-based transcriptional repression in yeast, delivered as a scoped design, strain build, and QC package.

Guide RNA design against your target promoter or coding region, reviewed before cloning.
DCas9-based repression constructs built in the yeast host background you specify.
Transcript-level repression assessment by qPCR with per-project controls when included.

Discuss Your Repression Project

What we need to scope your project

Share the yeast host, the target gene or promoter region, and the readout you plan to use, and we return a design and build plan for review before any laboratory work begins.

Yeast strain background plus any auxotrophic or selection markers already.
Target gene, promoter region, or pathway of interest
Preferred readout: transcript level, reporter signal.

dCas9 Repression

Yeast Hosts

qPCR Readout

Our Approach

Catalytically inactive Cas9 repression, built around your target

CRISPRi lowers transcription without cutting DNA, so the same yeast background can be carried into follow-on expression or pathway work.

Target and Guide Design

Every project starts by reviewing the target locus, the yeast host, and how partial repression will be read out. Guides are designed and reviewed against the chosen promoter or coding region before any construct is built, and the design notes are shared for confirmation.

Target locus and promoter region reviewed for repression suitability
Guide RNAs designed for the specified yeast host background
Design summary shared for customer review before cloning

Repression Constructs for the Chosen Host

Repression constructs are assembled for the yeast host in your project scope, with guide expression and dCas9 components carried on the vector format agreed in the SOW. Constructs are verified before they move into strain work.

Vector format and selection markers confirmed during scoping
Assembled constructs verified before transformation
Sequence-level construct records retained for handoff

No Double-Strand Break

Repression is achieved by blocking transcription rather than cutting DNA, which suits targets where a full deletion would not answer the question being asked.

Tunable by Design

Guide placement and expression strength are selected during design, so the repression window you need can be discussed and fixed in scope before work starts.

Reusable Backgrounds

Repression strains can be kept as glycerol stocks when included, which supports later expression, reporter, or pathway experiments in the same host.

Deliverables

What your project hands off

Handoff is a checklist rather than a promise of phenotype: what ships depends on the scope agreed in the SOW and on the target biology involved.

Materials and strains

Repression construct maps and sequence records for the vectors built
Yeast strains carrying the repression cassette.
Parent or non-targeting-guide control strains when included in scope
Guide RNA sequences and the plasmid set included when project-scoped

Data and documentation

Construct verification summary, including sequencing results for the assembled constructs.
Transcript-level repression data from the qPCR runs performed.
Growth or reporter readouts when those assays form part of the agreed scope.
Project report covering methods and assay conditions.

Service Scope

Parameters we fix with you, case by case

Scope is agreed after the target and host are reviewed, so the table below describes what can be customized rather than a fixed bundle.

Parameter	Typical project scope
Host / strain background	Saccharomyces cerevisiae is commonly requested; other yeast hosts are evaluated during scoping.
Target locus type	Promoter-proximal or coding-region targets, reviewed for suitablity before design begins.
Repression architecture	DCas9-based repression; effector, promoter, and guide-expression choices confirmed in the SOW.
Guide RNA scope	Single or multiple guides per target as scoped; combinatorial designs are quoted case by case.
Vector and marker format	Plasmid or integrating format selected to suit the host background and markers already in use.
Readout	Transcript-level assessment by qPCR; reporter or growth-based readouts when included in scope.
Controls	Non-targeting guide and untreated controls when included in the agreed project scope.
Strain screening depth	Repression-positive isolates prioritized for characterization; isolate counts defined in the SOW.
Technical support	Named scientific contact at project start; milestone review calls; email response within 1 business day

How Engagement Works

From target review to project handoff

A five-stage engagement that keeps design decisions visible before laboratory work moves forward.

Scoping and target review

We review the yeast host, target locus, and intended readout, then confirm which repression format, controls, and assay conditions fit the project before construct work begins.

Guide design and construct planning

Guide RNAs are designed against the agreed target region, and the vector, marker, and guide-expression strategy are fixed in a written design plan for customer review.

Construct assembly and assessment

Repression cassettes are assembled, then checked at the sequence level before they are released for yeast transformation and downstream strain work in the agreed background.

Strain work and repression assessment

Constructs are introduced into the agreed yeast background, candidate isolates are screened, and transcript-level repression is assessed under the conditions defined in scope.

Reporting and handoff

Results, construct records, and the agreed material set are compiled into a project report, with any follow-on options discussed at the milestone review call.

Typical Applications

Where yeast CRISPRi repression is commonly used

These are the project types customers most often bring to a repression campaign; fit for your target is confirmed during scoping.

Functional studies of essential or hard-to-delete genes

Repression lowers transcription without removing the locus, which supports investigation of genes where a deletion strain is not viable or not informative for the question at hand.

Partial repression of genes that cannot be cleanly deleted
Paired repression and control strains for comparative transcript work
Follow-on expression or reporter assays when project-scoped

Pathway and metabolic gene dosage studies

Tuning a pathway gene down rather than out can help investigate how expression level relates to the behaviour you are studying, with outcomes depending on the target and process conditions.

Multi-gene repression considered case by case during scoping
Conditions and sampling points agreed before assay work
Transcript data reported alongside the construct records

Candidate target and repression-window assessment

CRISPRi can be used to model partial inhibition of a target of interest and to investigate how much repression is needed before a readout changes in your system.

Graded or partial repression strategies when project-scoped
Readouts chosen to match the question being asked
Interpretation notes on what the data does and does not show

FAQ

Questions buyers ask before starting

Short answers on host choice, measurement, controls, and how scope is confirmed.

Saccharomyces cerevisiae is the most commonly requested background, and other yeast hosts are reviewed case by case during scoping, since guide expression, selection markers, and transformation conditions differ between species.
No cutting step is used by design. The catalytically inactive Cas9 binds the target region and blocks transcription, so the engagement is aimed at repression rather than editing; any downstream genome-editing work would be quoted as a separate scope.
Transcript levels are typically assessed by qPCR against the agreed controls, with reporter or growth readouts added when scoped. Acceptance criteria are set in the SOW, because the repression range achieved depends on the target, guide placement, and host background.
Repression is often chosen for essential genes because the locus is not removed. The design still has to be reviewed for your host, since guide placement and repression strength influence how much residual expression remains in the strain.
Constructs are sequence-verified before strain work, and non-targeting or parent controls are included when scoped. QC records, guide sequences, and assay conditions are compiled into the project report that accompanies the handoff materials.

Scientific Literature

References

Ishikawa K, Soejima S, Masuda F, et al. Implementation of dCas9-mediated CRISPRi in the fission yeast Schizosaccharomyces pombe. G3 (Bethesda, Md.). 2021;11(4). View on PubMed
Momen-Roknabadi A, Oikonomou P, Zegans M, et al. An inducible CRISPR interference library for genetic interrogation of Saccharomyces cerevisiae biology. Communications biology. 2020;3(1):723. View on PubMed
Arita Y, Kim G, Li Z, et al. A genome-scale yeast library with inducible expression of individual genes. Molecular systems biology. 2021;17(6):e10207. View on PubMed
Ishikawa K, Saitoh S. Transcriptional Regulation Technology for Gene Perturbation in Fission Yeast. Biomolecules. 2023;13(4). View on PubMed
Misa J, Schwartz C. CRISPR Interference and Activation to Modulate Transcription in Yarrowia lipolytica. Methods in molecular biology (Clifton, N.J.). 2021;2307:95-109. View on PubMed

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