Catalog Number | Product Size | Concentration | Price |
---|---|---|---|
GE0010S | 70 pmol | 1 μM | Online Inquiry |
GE0010L | 400 pmol | 20 μM | Online Inquiry |
Product Information | |
---|---|
Description | Staphylococcus aureus Cas9 is expressed as a C-terminal 6×His-tagged fusion in E. coli. It is a programmable DNA endonuclease guided by a sgRNA. Staphylococcus aureus Cas9 can catalyze site-specific cleavage of double-stranded DNA. A ~100 nucleotide gRNA complementary to the target site and a 5′ NNGRRT 3′ (N = any, R = A or G) protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence are required for targeting. |
Applications | Random Mutagenesis |
Properties | |
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Storage Temperature | -20°C |
Storage Conditions | • 20 mM Tris-HCl • 300 mM NaCl • 0.1 mM TCEP • 50% Glycerol • pH 7.5 at 25°C |
Shipping Conditions | Blue ice. |
Heat Activation | 65°C for 5 min |
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