CRISPR/Cas9 Genome Editing in Saccharomyces cerevisiae

We routinely perform precise knockouts (disruption or full ORF deletion), point mutations, small insertions (e.g., epitope tags), and sequence replacements. The editing strategy is designed per project to evaluate success using homologous recombination with a custom donor template. More complex edits such as large deletions or integrations can be scoped as needed.

Every project includes colony PCR screening of candidate clones followed by Sanger sequencing of the edited region and flanking boundaries to confirm the intended modification. A sequencing alignment comparing the edited sequence to the parent strain is provided. Off-target assessment is performed in silico during guide RNA design, and optional targeted amplicon sequencing can be added by scope.

Yes, we have experience with a variety of laboratory and industrial Saccharomyces cerevisiae strains. Polyploid and aneuploid strains require additional screening to edit all alleles; we will discuss the strategy and expected success rate during the design phase. Custom transformation optimization may be applied as needed.

You provide the target gene name or sequence (or genomic coordinates), the desired edit type, and the strain background. For knock-ins, please provide the sequence to be inserted or the tag to be added. Our team will then propose a detailed strategy including guide RNA and donor design for your review before work begins.