Catalog Number | Product Size | Concentration | Price |
---|---|---|---|
GE0003S | 70 pmol | 1 μM | Online Inquiry |
GE0003L | 400 pmol | 20 μM | Online Inquiry |
Product Information | |
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Description | CD® Cas9 Nickase is expressed as a N-terminal 6×His-tagged fusion in E. coli. It is a variant of Cas9 nuclease. CD® Cas9 Nickase has a point mutation (D10A) in the RuvC nuclease domain and is able to generate target-specific nicks with reduced off-target effects. |
Applications | Random Mutagenesis |
Properties | |
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Storage Temperature | -20°C |
Storage Conditions | • 300 mM NaCl • 10 mM Tris-HCl • 0.1 mM EDTA • 1 mM DTT • 50% Glycerol • pH 7.4 at 25°C |
Shipping Conditions | Blue ice. |
Heat Activation | 65°C for 5 min |
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