Synthetic Biology
CD<sup>®</sup> Cas9 Nickase

CD® Cas9 Nickase

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CD® Cas9 Nickase

Catalog Number Product Size Concentration Price
GE0003S 70 pmol 1 μM Online Inquiry
GE0003L 400 pmol 20 μM Online Inquiry
Product Information
Description CD® Cas9 Nickase is expressed as a N-terminal 6×His-tagged fusion in E. coli. It is a variant of Cas9 nuclease. CD® Cas9 Nickase has a point mutation (D10A) in the RuvC nuclease domain and is able to generate target-specific nicks with reduced off-target effects.
Applications Random Mutagenesis
Properties
Storage Temperature -20°C
Storage Conditions • 300 mM NaCl
• 10 mM Tris-HCl
• 0.1 mM EDTA
• 1 mM DTT
• 50% Glycerol
• pH 7.4 at 25°C
Shipping Conditions Blue ice.
Heat Activation 65°C for 5 min

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