Product Information | |
---|---|
Product Name | CD Carbonyl Assay Kit |
Applications | For quantitative determination of carbonyl groups or protein carbonyls and evaluation of drug modulators. |
Features | Fast and sensitive. Use of 100 µL sample. Linear detection range from 12 to 250 µM carbonyl in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 min. High-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. |
Detection Method | OD375nm |
Sample Type | Chemicals with carbonyl groups (e.g. ketones, aldehydes) or protein carbonyls in biological samples (e.g. oxidized BSA, etc.) |
Species | All |
Assay Time | 30 min |
Limit of Detection | 12 µM |
Expiration Date | 12 months |
Description | CARBONYL groups, such as ketones and aldehydes, are a commonindicator of protein oxidation. Protein oxidation is caused as a result of exposure to reactive oxygen species (ROS) and is a common marker in various diseases as well as aging. BioAssay Systems’ Carbonyl Assay Kit is based on an improved method, where 2,4-dinitrophenylhydrazine (DNPH) reacts with carbonyl groups to produce a colored compound at 375 nm. The intensity of this colored compound is directly proportional to the carbonyl groups in the sample. |
Shipping Conditions | Shipping: RT |
Storage | Storage: -20°C upon receipt |
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.