CD Aconitase Assay Kit

Catalog Number: AKBA115

Size: 100 tests

Product Information
Product Name	CD Aconitase Assay Kit
Applications	For quantitative determination of aconitase enzyme activity and high-throughput screening assays for aconitase modulator.
Features	Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 100 U/L for 20 min reaction. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	OD565nm
Sample Type	Biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)
Species	All
Assay Time	30 min
Limit of Detection	0.5 U/L
Expiration Date	6 months
Description	ACONITASE (ACONITATE HYDRATASE) is an enzyme in the citric acid (TCA) cycle that catalyzes the conversion of citrate to isocitrate. The activity of aconitase depends largely upon the iron-sulfur [Fe4S4] 2+ cluster. Related diseases include aconitase deficiency (e.g. myopathy and exercise intolerance), Friedreich’s ataxia, and diabetes. BioAssay Systems’ aconitase assay measures the isocitrate generated as a product of the aconitase reaction. The isocitrate is then oxidized producing NADPH and the oxidation product. The NADPH converts the dye to an intense violet color with an absorption maximum of 565 nm. The increase in absorbance at 565 nm is directly proportional to aconitase activity.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

Q & A

Customer Reviews

Datasheet

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

First Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Product Review Title *

Summary *

First Name

Country *

Email *

Datasheet

Please note that all services are for research use only. Not intended for any clinical use.

CD Aconitase Assay Kit