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SPEG Knockout Cell Lines

Gene: SPEG

Official Full Name: striated muscle enriched protein kinaseprovided by HGNC

Gene Summary: This gene encodes a protein with similarity to members of the myosin light chain kinase family. This protein family is required for myocyte cytoskeletal development. Along with the desmin gene, expression of this gene may be controlled by the desmin locus control region. Mutations in this gene are associated with centronuclear myopathy 5. [provided by RefSeq, Jun 2016]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO32000 SPEG Knockout cell line (HeLa) Human SPEG 1:3~1:6 Negative Online Inquiry
KO32001 SPEG Knockout cell line (HCT 116) Human SPEG 1:2~1:4 Negative Online Inquiry
KO32002 SPEG Knockout cell line (HEK293) Human SPEG 1:3~1:6 Negative Online Inquiry
KO32003 SPEG Knockout cell line (A549) Human SPEG 1:3~1:4 Negative Online Inquiry

Background

SPEG Gene Knockout Cell Lines are a meticulously engineered biological tool that allows researchers to study the functional role of the SPEG gene through targeted gene disruption. These cell lines utilize the CRISPR-Cas9 gene editing technology, which enables precise modifications within the cell’s genomic DNA, leading to the knockout of the SPEG gene. This intentional disruption facilitates in-depth analyses of the gene's biological pathways, cellular processes, and potential implications in various physiological and pathological conditions.

The mechanism of action for SPEG Gene Knockout Cell Lines hinges on the utilization of guide RNA sequences that specifically direct the Cas9 nuclease to the region flanking the SPEG gene. Once introduced into the cell, Cas9 induces double-strand breaks, which are then repaired by the cell's native homology repair mechanisms, leading to insertions or deletions that effectively disrupt the gene's coding region. This targeted gene knockout is pivotal for elucidating the gene's function and its involvement in cellular processes such as signaling pathways, muscle development, and cardiac health.

In the realm of research and clinical settings, SPEG Gene Knockout Cell Lines serve as invaluable resources for exploring gene function, understanding disease mechanisms, and developing potential therapeutic strategies. Scientists can leverage these cell lines to model diseases related to the cardiovascular system, thereby advancing our understanding of their underlying genetics and pathophysiology.

What sets SPEG Gene Knockout Cell Lines apart from conventional alternatives is their high specificity and efficiency in gene targeting, along with robust validation of knockout efficacy. Researchers benefit from this enhanced precision, which minimizes off-target effects and maximizes experimental reproducibility. These attributes underscore the product's utility in high-stakes experimental environments where accuracy is paramount.

For researchers and clinicians alike, the value of SPEG Gene Knockout Cell Lines is immense, facilitating breakthroughs in gene function studies and paves the way for novel insights into disease mechanisms that could translate into impactful therapies. Our company is committed to providing cutting-edge biological tools, backed by extensive expertise and a strong focus on pioneering advancements in genetic research, positioning us as a leader in the biotechnological landscape.

Please note that all services are for research use only. Not intended for any clinical use.

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