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LAMA4 Knockout Cell Lines

Gene: LAMA4

Official Full Name: laminin subunit alpha 4provided by HGNC

Gene Summary: Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the alpha chain isoform laminin, alpha 4. The domain structure of alpha 4 is similar to that of alpha 3, both of which resemble truncated versions of alpha 1 and alpha 2, in that approximately 1,200 residues at the N-terminus (domains IV, V and VI) have been lost. Laminin, alpha 4 contains the C-terminal G domain which distinguishes all alpha chains from the beta and gamma chains. The RNA analysis from adult and fetal tissues revealed developmental regulation of expression, however, the exact function of laminin, alpha 4 is not known. Tissue-specific utilization of alternative polyA-signal has been described in literature. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Aug 2011]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO10266 LAMA4 Knockout cell line (HeLa) Human LAMA4 1:3~1:6 Negative Online Inquiry
KO10267 LAMA4 Knockout cell line (HCT 116) Human LAMA4 1:2~1:4 Negative Online Inquiry
KO10268 LAMA4 Knockout cell line (HEK293) Human LAMA4 1:3~1:6 Negative Online Inquiry
KO10269 LAMA4 Knockout cell line (A549) Human LAMA4 1:3~1:4 Negative Online Inquiry

Background

LAMA4 Gene Knockout Cell Lines are specialized cellular models engineered to have a targeted disruption of the LAMA4 gene, which encodes for the laminin α4 chain, a crucial component of the extracellular matrix. This innovative product allows researchers to investigate the specific functions of LAMA4 in cellular processes such as adhesion, migration, and differentiation. By using CRISPR-Cas9 technology or other gene-editing techniques, these cell lines enable precise modulation of gene expression, facilitating the study of LAMA4’s role in various biological pathways and disease models.

The key functions of the LAMA4 Gene Knockout Cell Lines are rooted in their ability to mimic pathogenic states by losing specific biochemical activities associated with the LAMA4 protein. Researchers can observe how alterations in the extracellular matrix affect cellular behavior, impacting studies related to cancer metastasis, wound healing, and tissue engineering. Moreover, these cell lines serve as vital tools in drug screening and mechanistic studies, providing insights into potential therapeutic targets for diseases linked to laminin signaling dysfunction.

In terms of scientific importance, LAMA4 Gene Knockout Cell Lines represent a powerful resource for both academic and clinical research settings. They have the potential to elucidate the roles of LAMA4 in pathological processes, thereby advancing our understanding of conditions such as fibrosis, neurological disorders, and vascular diseases. Unlike traditional cell lines, these knockout models offer a more accurate representation of how cellular environments are affected by the absence of specific proteins, leading to more reliable translational research outcomes.

The unique selling points of LAMA4 Gene Knockout Cell Lines include their high specificity in targeting the LAMA4 gene, robustness in experimental applications, and the ability to provide reproducible results across diverse cell types. Furthermore, the availability of these knockout models accelerates the research timeline, allowing investigators to derive significant findings with reduced experimental variability.

For researchers and clinicians seeking to understand the complex roles of the extracellular matrix in health and disease, LAMA4 Gene Knockout Cell Lines stand as an invaluable resource. They offer the promise of contributing to groundbreaking discoveries that may lead to new therapeutic strategies.

Our company prides itself on its extensive experience in the field of genetic engineering and cell biology, ensuring that our products meet the highest standards of quality and reliability. With a commitment to supporting the scientific community, we strive to provide cutting-edge tools that empower researchers to push the boundaries of knowledge in biological sciences.

Please note that all services are for research use only. Not intended for any clinical use.

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