HLA-A Knockout Cell Lines

Gene: HLA-A

Official Full Name: major histocompatibility complex, class I, Aprovided by HGNC

Gene Summary: HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen so that they can be recognized by cytotoxic T cells. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. More than 6000 HLA-A alleles have been described. The HLA system plays an important role in the occurrence and outcome of infectious diseases, including those caused by the malaria parasite, the human immunodeficiency virus (HIV), and the severe acute respiratory syndrome coronavirus (SARS-CoV). The structural spike and the nucleocapsid proteins of the novel coronavirus SARS-CoV-2, which causes coronavirus disease 2019 (COVID-19), are reported to contain multiple Class I epitopes with predicted HLA restrictions. Individual HLA genetic variation may help explain different immune responses to a virus across a population.[provided by RefSeq, Aug 2020]

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Products Background

Products

Catalog Number	Product Name	Species	Gene	Passage ratio	Mycoplasma testing	Price
KO17107	HLA-A Knockout cell line (HeLa)	Human	HLA-A	1:3~1:6	Negative	Online Inquiry
KO17108	HLA-A Knockout cell line (HCT 116)	Human	HLA-A	1:2~1:4	Negative	Online Inquiry
KO17109	HLA-A Knockout cell line (HEK293)	Human	HLA-A	1:3~1:6	Negative	Online Inquiry
KO17110	HLA-A Knockout cell line (A549)	Human	HLA-A	1:3~1:4	Negative	Online Inquiry

Background

HLA-A Gene Knockout Cell Lines are specialized cell cultures that have been genetically modified to eliminate the expression of the human leukocyte antigen A (HLA-A) gene. This innovative tool is integral for studying immune responses, particularly in the context of transplantation biology, autoimmune diseases, and cancer immunotherapy. By knocking out the HLA-A gene, researchers can create a controlled environment to investigate the role of HLA-A in T-cell recognition, antigen presentation, and overall immune evasion mechanisms.

The functionality of these knockout cell lines is rooted in the precise CRISPR-Cas9 gene-editing technology, which allows for targeted modification of specific genomic sequences. This method not only ensures high specificity but also facilitates the generation of consistent and reproducible models for experimentation. In clinical and research settings, HLA-A knockout cell lines serve as vital platforms to evaluate cellular responses and the efficacy of immune-based therapies, as they eliminate confounding variables related to HLA-mediated antigen presentation.

The scientific importance of HLA-A Gene Knockout Cell Lines lies in their applicability across a range of disciplines, including immunology, therapeutic vaccine development, and cancer research. These models enable researchers to elucidate mechanisms of immune evasion in tumors and explore novel avenues for enhanced immunotherapy strategies. Compared to traditional cell lines that express HLA molecules, these knockout lines provide a more refined approach for dissecting the immune landscape without the interference of HLA antigen variability.

The distinct advantages of HLA-A Gene Knockout Cell Lines include the reduction of variable HLA expression, enabling clearer interpretation of experimental results. Additionally, they are specifically curated to facilitate high-throughput screening processes, making them efficient for large-scale studies. Researchers and clinicians will find these knockout cell lines invaluable for deciphering the complexities of immune responses, thereby paving the way for breakthroughs in personalized medicine.

Our company stands at the forefront of biotechnological innovation, offering expertly crafted genetic tools like HLA-A Gene Knockout Cell Lines. We are dedicated to supporting the scientific community with high-quality products that empower transformative research in immunology and beyond.

Please note that all services are for research use only. Not intended for any clinical use.

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