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GNAS Knockout Cell Lines

Gene: GNAS

Official Full Name: GNAS complex locusprovided by HGNC

Gene Summary: This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. [provided by RefSeq, Aug 2012]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
GP00234 GNAS gRNA1-gRNA2 KO plasmid GNAS $850
GP00513 GNAS gRNA3-gRNA4 KO plasmid GNAS $850
KO00783 GNAS Knockout cell line(HeLa) Human GNAS 1:3~1:6 Negative Online Inquiry
KO08815 GNAS Knockout cell line (HCT 116) Human GNAS 1:2~1:4 Negative Online Inquiry
KO08816 GNAS Knockout cell line (HEK293) Human GNAS 1:3~1:6 Negative Online Inquiry
KO08817 GNAS Knockout cell line (A549) Human GNAS 1:3~1:4 Negative Online Inquiry

Background

GNAS Gene Knockout Cell Lines are genetically modified cells in which the GNAS gene, known for its role in the regulation of hormone signaling and energy metabolism, has been selectively disrupted. This innovative product allows researchers to study the specific biological functions of the GNAS gene in cellular contexts that mimic physiological conditions, providing insights into the gene's role in various pathologies, including metabolic disorders and endocrine diseases.

The primary function of GNAS Gene Knockout Cell Lines involves the examination of gene function and signaling pathways that are under the transcriptional control of GNAS. When GNAS is knocked out, the resulting alterations in downstream signaling cascades, such as the cAMP pathway, enable researchers to understand the gene's impact on cellular processes like proliferation, differentiation, and metabolism. This manipulation facilitates high-throughput screening and functional assays, rendering it a versatile tool for elucidating gene function in both basic and applied biological research.

The scientific importance of these knockout models extends to their application in drug discovery, genetic studies, and disease modeling, providing a platform for evaluating the efficacy of therapeutic strategies targeting GNAS-related pathways. Researchers in academia and industry can leverage these cell lines to gain deeper insights into the molecular mechanisms underlying diseases associated with GNAS dysfunction, thus fostering the development of innovative treatments.

Among the advantages of GNAS Gene Knockout Cell Lines is their specificity and reliability, which surpass many conventional models that may exhibit off-target effects or genetic variability. This specificity enhances the reproducibility of experimental results, ensuring researchers can draw more robust conclusions. Furthermore, the availability of these cell lines in multiple relevant backgrounds allows for greater flexibility in experimental designs.

The GNAS Gene Knockout Cell Lines represent a valuable tool for researchers, offering unparalleled insights into the biological significance of a critical gene. By integrating these cell lines into their workflows, scientists can accelerate discovery efforts and enhance their understanding of complex biological systems. Our company prides itself on delivering high-quality, cutting-edge genetic models, backed by rigorous validation and extensive expertise in genetic engineering, making us a trusted partner for your research endeavors.

Please note that all services are for research use only. Not intended for any clinical use.

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