Product Information | |
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Product Name | CD Phosphoglucomutase Assay Kit |
Applications | For quantitative determination of phosphoglucomutase enzyme activity in biological samples. |
Features | Safe and sensitive. Non-radioactive assay. Linear detection range in 96-well plate: 0.5 to 20 U/L activity. Fast and convenient. The procedure involves addition of a single working reagent and incubation for 20 min. Room temperature assay. No 37°C incubator is needed. High-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day. |
Detection Method | OD460nm |
Sample Type | Serum, plasma, cell lysate, and other biological samples |
Species | All |
Assay Time | 20 min |
Limit of Detection | 0.5 U/L |
Expiration Date | 6 months |
Description | PHOSPHOGLUCOMUTASE (E.C. 5.4.2.2) is an enzyme which catalyzes the interconversion of glucose-1-phosphate (G1P) and glucose-6-phosate (G6P). Phosphoglucomutase (PGM) plays a crucial role in glycogen synthesis and degradation. PGM 1 deficiency can cause a rare congenital disorder of glycosylation. A decrease in PGM activity has also been linked with oxidative stress. In BioAssay Systems’ EPGM-100 assay, PGM converts G1P to G6P, which is then oxidized by glucose-6-phosphate dehydrogenase. The formed NADPH is coupled to a formazan chromogen. The increase in absorbance at 460 nm is directly proportional to phosphoglucomutase activity. |
Storage | Storage: -20°C upon receipt. |
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