CD Nitric Oxide Synthase Inhibitor Screening Kit

Catalog Number: AKBA486

Size: 100 tests

(AKBA486)CD Nitric Oxide Synthase Inhibitor Screening Kit

Product Information
Product Name	CD Nitric Oxide Synthase Inhibitor Screening Kit
Applications	For evaluation and high-throughput screen (HTS) of nitric oxide synthase (NOS) modulators.
Features	High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling system. Rapid and reliable. Can be completed in less than 3 hours if the assay is performed at 37°C.
Detection Method	OD540nm
Sample Type	Nitric Oxide Synthase
Species	All
Assay Time	2-3 hours
Expiration Date	6 months
Description	Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO is the oxidation product of arginine by nitric oxide synthase (NOS) and is involved in host defense development, activation of regulatory proteins, and direct covalent interaction with functional biomolecules. Inhibition of NOS has the potential to produce diverse biological effects, particularly in the cardiovascular system. Simple, direct, and non-radioactive procedures for measuring NOS are becoming popular in research and drug discovery. BioAssay Systems EnzyChrom™ Nitric Oxide Synthase Inhibitor Assay Kit involves two steps: a NOS reaction step during which NO is produced followed by an NO detection step. Since the NO generated by NOS is rapidly oxidized to nitrite and nitrate, the NO production is measured following the reduction of nitrate to nitrite using an improved Griess method.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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CD Nitric Oxide Synthase Inhibitor Screening Kit