Product Information | |
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Product Name | CD NAD/NADH Assay Kit |
Applications | For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism. |
Features | Sensitive and accurate. The detection limit of 0.05 µM and linearity up to 10 µM NAD+/NADH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 15 min at room temperature. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. |
Detection Method | OD565nm |
Sample Type | Cell or tissue extracts |
Species | All |
Assay Time | 15 min |
Limit of Detection | 0.05 µM |
Expiration Date | 6 months |
Description | Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD+/NADH has applications in research pertaining to energy transformation and the redox state of cells or tissue. Simple, direct, and automation-ready procedures for measuring NAD+/NADH concentration are very desirable. BioAssay Systems EnzyChrom™ NAD+/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportional to the NAD+/NADH concentration in the sample. This assay is highly specific for NAD+/NADH and with minimal interference (<1%) by NADP+/NADPH. Our assay is a convenient method to measure NAD, NADH, and their ratio. |
Shipping Conditions | Shipping: On Ice |
Storage | Storage: -20°C upon receipt |
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