CD α-Mannosidase Assay Kit

Catalog Number: AKBA225

Size: 100 tests

Product Information
Product Name	CD α-Mannosidase Assay Kit
Applications	For quantitative determination of α-mannosidase enzyme activity.
Features	Fast and sensitive. Linear detection range (10 µL sample): 1 to 250 U/L for a 10-minute reaction. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	OD405nm
Sample Type	Biological samples e.g. plasma, serum, tissue, and culture media
Species	All
Assay Time	10 min
Limit of Detection	1 U/L
Expiration Date	6 months
Description	α-MANNOSIDASE (AMA) is an enzyme that catalyzes the cleavage of the α form of mannose. α-Mannosidase assists in the breakdown of complex sugars from glycoproteins in the lysosome. Defective AMA or deficient AMA activity causes α-mannosidosis and leads to deterioration of the central nervous system in children. BioAssay Systems’ non-radioactive, colorimetric AMA assay is based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after the addition of the stop reagent. The increase in absorbance at 405 nm after the addition of the stop reagent is directly proportional to the enzyme activity.
Shipping Conditions	Shipping: RT
Storage	Storage: 4°C upon receipt

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CD α-Mannosidase Assay Kit