Product Information | |
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Product Name | CD L-Lactate Assay Kit |
Applications | For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism. |
Features | Sensitive and accurate. The detection limit of 0.05 mM and linearity up to 2 mM L-Lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM L-Lactate in a 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. |
Detection Method | OD565nm |
Sample Type | Serum, plasma, cell culture media, etc |
Species | All |
Assay Time | 20 min |
Limit of Detection | 0.05 mM |
Expiration Date | 6 months |
Description | Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct, and automation-ready procedures for measuring lactate concentration are very desirable. BioAssay Systems EnzyChrom™ lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample. |
Shipping Conditions | Shipping: On Ice |
Storage | Storage: -20°C upon receipt |
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