CD Isocitrate Assay Kit

Catalog Number: AKBA134

Size: 100 tests

Product Information
Product Name	CD Isocitrate Assay Kit
Applications	For quantitative colorimetric determination of isocitrate and evaluation of drug effects on its metabolism.
Features	Fast and sensitive. Linear detection range (20 µL sample): 20 to 5000 µM for 10 min reaction. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	OD565nm
Sample Type	Food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)
Species	All
Assay Time	10 min
Limit of Detection	20 µM
Expiration Date	6 months
Description	ISOCITRATE (ISOCITRIC ACID) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing α-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices. BioAssay Systems’ isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the isocitrate concentration
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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CD Isocitrate Assay Kit