Product Information | |
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Product Name | CD Glutathione Reductase Kit |
Applications | For quantitative determination of glutathione reductase activity. |
Features | Fast and sensitive. Linear detection range (20 µL sample): 0.4 to 50 U/L for 20 min reaction. Detection Limit of 0.4 U/L. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. |
Detection Method | OD412nm |
Sample Type | Plasma, serum, tissue and culture media etc |
Species | All |
Assay Time | 30 min |
Limit of Detection | 0.4 U/L |
Expiration Date | 6 months |
Description | GLUTATHIONE REDUCTASE (GR) reduces oxidized glutathione (GSSG) to the reduced sulfhydryl form GSH which is an important cellular antioxidant. A high GSH/GSSG ratio is important for protection against oxidative stress. Thus, measurement of GR activity is used as indicator for oxidative stress. BioAssay Systems’ non-radioactive, colorimetric GR assay is designed to accurately measure GR activity in biological samples with a method that utilizes Ellman’s method in which DTNB reacts with the GSH generated from the reduction of GSSG by the GR in a sample to form a yellow product (TNB2-). The rate of change in the optical density, measured at 412 nm, is directly proportional to GR activity in the sampl |
Shipping Conditions | Shipping: On Ice |
Storage | Storage: -20°C upon receipt |
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