Product Information | |
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Product Name | CD Caspase-3 Assay Kit |
Applications | For quantitative determination of the apoptosis target caspase-3 activity and HTS screen for apoptosis inducers and inhibitors. |
Features | Safe. Non-radioactive assay. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. |
Detection Method | FL400/490nm |
Sample Type | Cell and tissue lysate |
Species | All |
Assay Time | 60 min |
Expiration Date | 12 months |
Description | Caspases are members of the aspartate-specific cysteinyl protease family that play a central role in apoptosis. Apoptosis is involved in a variety of physiological and pathological events, ranging from normal fetal development to diseases such as cancer, organ failure, and neurodegenerative diseases. Caspase-3 is key biomarker in the assessment of apoptosis and in understanding mechanism of apoptosis induction. BioAssay Systems Caspase-3 Assay Kit provides a convenient means to measure caspase-3 activity in biological samples. In the assay, a specific substrate (N-Ac-DEVD-AFC) is cleaved by active caspase-3, forming a highly fluorescent product.The fluorescence intensity (λex/em = 400/490nm) is proportional to the caspase-3 activity. |
Shipping Conditions | Shipping: RT |
Storage | Storage: -20°C upon receipt |
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