CD Biotin Assay Kit

Catalog Number: AKBA285

Size: 100 tests

Price: $717

Product Information
Product Name	CD Biotin Assay Kit
Applications	For quantitative determination of biotin in food, cosmetic, and biotinylated proteins.
Features	Fast and sensitive. Linear detection range: 8 to 200 µM biotin with 30 µL sample (96-well) or 10 to 200 µM biotin with 10 µL sample (384-well). Convenient. The procedure involves adding a single working reagent and reading after 20 minutes. High-throughput. “Add-mix-read” type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day.
Detection Method	OD500nm
Sample Type	Food, cosmetics, supplements, and biotinylated proteins or antibodies
Species	All
Assay Time	20 min
Limit of Detection	8 µM
Expiration Date	12 months
Description	BIOTIN , or Vitamin B7, is a water soluble vitamin involved in metabolism, cell growth, and protein synthesis. It is a cofactor to multiple carboxylases necessary for metabolizing fatty acids, glucose, and amino acids. Biotin is found in a wide range of foods and is often taken as a dietary supplement. In the biotechnology industry, biotin is commonly conjugated to proteins in a process called biotinylation. These biotinylated proteins can then be specifically selected using streptavidin and/or avidin’s strong affinity for biotin in biochemical assays such as ELISAs.BioAssay Systems’ Biotin Assay Kit is based on avidin’s weak affinity for 4′-hydroxyazobenzene-2-carboxylic acid (HABA) and strong affinity for biotin. The avidin-HABA complex reagent has an absorbance at 500nm. When the colored avidin-HABA reagent is introduced to biotin, the biotin binds to avidin, displacing the HABA and causing a decrease in absorbance. The decrease in absorbance at 500nm is directly proportional to the biotin in the sample.
Shipping Conditions	Shipping: RT
Storage	Storage: -20°C upon receipt

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CD Biotin Assay Kit