Algal Metabolic Engineering Services

We have extensive experience with Chlamydomonas reinhardtii (a model green alga) and several Nannochloropsis species (especially N. oceanica and N. gaditana). Other phototrophic microalgae, such as Phaeodactylum tricornutum or Haematococcus pluvialis, can be accommodated after scoping. We will help you select the best chassis for your target molecule.

Stability is assessed by serially passaging the best clones under non‑selective conditions (light, CO₂, minimal medium) for generations. We re‑measure product yield and confirm transgene presence by PCR or sequencing. Only strains that maintain ≥ project-dependent performance that is verified during development of the initial phenotype are included in the final deliverable. Polyploid / multi‑nucleate hosts (e.g., Chlamydomonas) are flagged, and stable integration is verified.

Absolute yields depend heavily on the target, the host strain, and cultivation conditions (photobioreactor vs shake flask). Our engineering approach aims to achieve industrially relevant improvements, but we cannot guarantee a specific fold‑increase or final titer without a preliminary feasibility study. Acceptance criteria are defined in the project SOW based on your baseline data.

Yes, we include in‑silico off‑target prediction for CRISPR‑Cas9 designs. Targeted amplicon sequencing of predicted off‑target loci can be added as a scoped option. We do not currently perform genome‑wide off‑target profiling (e.g., GUIDE‑seq) for algae, as such assays are not validated for these hosts. Off‑target analysis depth is defined at the project scoping stage.

Most projects are completed a timeline confirmed during project scoping. Complex projects involving knock‑in of large constructs, multi‑gene pathways, or extensive clone screening may take longer. A detailed timeline is provided in the project proposal before work begins.