Surface Biotinylation Service

Sulfo-NHS-Biotin forms a permanent, non-cleavable amide bond with primary amines. Sulfo-NHS-SS-Biotin contains a cleavable disulfide bond in the spacer, allowing biotin to be released under reducing conditions if needed for downstream applications.

Choose shorter spacers (13.5A) when minimal steric hindrance is critical. Choose longer spacers (22.4A-30.5A) when improved accessibility for streptavidin binding or downstream applications is needed. PEG spacers provide better solubility and reduced aggregation.

Yes. Our membrane-impermeant Sulfo-NHS reagents are specifically designed for live cell labeling. The negatively charged sulfo group prevents membrane penetration, ensuring only extracellular proteins are labeled while maintaining cell viability.

Every batch undergoes HABA assay to quantify biotin incorporation, mass spectrometry verification to confirm molecular weight, and SDS-PAGE analysis to assess labeling efficiency. Detailed QC reports are included with each delivery.

The optimal pH range is 7.5-8.5. The reaction proceeds efficiently from pH 7.0-9.0. Lower pH reduces reactivity while higher pH may cause protein degradation. We recommend using fresh reagents and maintaining consistent pH throughout the reaction.