RASGRF1 Knockout cell line (HCT 116)

Catalog Number: KO10516

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Specifications

Product Information
Product Name	RASGRF1 Knockout cell line (HCT 116)
specification	1*10^6
Storage and transportation	Dry ice preservation/T25 live cell transportation.
Cell morphology	Epithelioid, adherent cell
Passage ratio	1:2~1:4
species	Human
Gene	RASGRF1
Gene ID	5923
Build method	Electric rotation method / virus method
Mycoplasma testing	Negative
Cultivation system	90%McCOYs 5A+10% FBS
Parental Cell Line	HCT 116
Quality Control	Genotype: RASGRF1 Knockout cell line (HCT 116) >95% viability before freezing. All cells were tested and found to be free of bacterial, viruses,mycoplasma and other toxins.

Gene Information
Gene Official Full Name	Ras protein specific guanine nucleotide releasing factor 1provided by HGNC
Also known as	GNRP; GRF1; CDC25; GRF55; CDC25L; H-GRF55; PP13187; ras-GRF1
Gene Description	The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Mar 2009]
Expression	Biased expression in brain (RPKM 10.4) and lung (RPKM 4.4) See more

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