CD Biosynsis offers accelerated Vibrio natriegens Strain Development and Screening Services, utilizing the host’s ultra-fast growth rate to significantly speed up the Design-Build-Test-Learn (DBTL) cycle for industrial biotechnology. V. natriegens is the fastest growing non-pathogenic organism known, with a doubling time of approximately 10 min, making it ideal for rapid strain construction and large-scale fermentation. Our services combine advanced genetic engineering tools (CRISPR-Cas9, Base Editing, CRISPRi) with automated high-throughput screening technologies (HTS) to quickly generate, evaluate, and optimize thousands of genetic variants. We specialize in engineering V. natriegens for enhanced yield, improved tolerance to industrial conditions, and efficient utilization of various feedstocks, providing a fast track from concept to commercial-ready production strain.
Get a QuoteThe speed of V. natriegens directly translates into rapid strain evolution and testing, allowing us to complete multiple cycles of strain optimization in the time typically required for a single cycle in slower hosts. Our comprehensive platform minimizes the bottleneck inherent in the Test phase of DBTL. We employ automated liquid handling and miniaturized culture formats to screen thousands of engineered clones per day. This integration of rapid Build (using CRISPR-based tools) and rapid Test (using HTS) is key to quickly achieving commercially viable production titers.
CRISPR-Cas9 System
Used for large insertions (pathway integration) or clean gene knockouts on either Chromosome I or Chromosome II of V. natriegens.
Base Editing (BE)
For high-precision, single-nucleotide substitutions to fine-tune regulatory elements (RBS/promoters) or optimize enzyme function.
CRISPR Interference (CRISPRi)
Tunable gene knockdown used for rapid identification of optimal flux balances in native or heterologous pathways.
Automated Microplate Culture
Using 96- or 384-well plates with automated liquid handling and robotic incubation for high-density strain library cultivation.
Microdroplet or FACS Screening
Advanced screening techniques enabling the ultra-high-throughput evaluation of up to $10^7$ cells per hour, coupled with fluorescence or absorbance detection.
Advanced Sensor Assays
Implementation of biosensors or coupled enzyme assays for the rapid, real-time detection and quantification of target products directly in the culture media.
Pathway Integration
Stable chromosomal insertion of complete heterologous biosynthetic pathways, ensuring non-loss during rapid cell division.
Host Tolerance Engineering
Modifying the strain to enhance tolerance to high product titers, osmotic stress, or inhibitory compounds found in industrial feedstocks.
Feedstock Utilization
Engineering native or expressing foreign transporters and metabolic enzymes to enable efficient utilization of diverse, low-cost carbon sources (e.g., glycerol, lignocellulose derivatives).
Integrated DBTL cycle for rapid, iterative strain optimization.
1. Design (Modeling & Target Identification)
2. Build (Rapid Genetic Editing)
3. Test (High-Throughput Screening)
4. Learn (Data Analysis & Iteration)
Computational modeling predicts optimal genetic modifications (knockouts, overexpression, tuning).
Design gRNAs/primers and synthetic DNA parts (promoter/RBS libraries).
Plan multiplexed or combinatorial editing strategies based on model output.
Construct high-diversity strain libraries using CRISPR tools (Cas9, BE, CRISPRi).
Execute rapid genome editing and chromosomal pathway integration in V. natriegens.
Verify genotype of the initial library population.
Analyze HTS data to correlate genotype with desired phenotype (yield/growth).
Refine the computational model and design the next, more focused set of genetic edits.
Deliver the final optimized production strain and complete strain history documentation.
Ultra-Fast Iteration
The host's 10-minute doubling time drastically shortens the time required for the Build and Test phases, enabling more DBTL cycles and faster strain improvement than standard hosts.
Integrated Toolset
Combines permanent editing (CRISPR-Cas9/BE) with tunable control (CRISPRi), allowing for both robust pathway installation and fine-tuning of metabolic flux.
High-Throughput Capability
Automated HTS systems are specifically adapted for V. natriegens's rapid growth kinetics, allowing the screening of thousands of variants in parallel for maximum coverage.
Stable Integration Focus
Expertise in achieving stable, dual-chromosome integration of large pathways, essential for preventing plasmid loss and ensuring long-term stability during industrial scale-up.
1. What makes V. natriegens superior to E. coli for strain development?
V. natriegens's 10-minute doubling time allows us to generate biomass and test variants twice as fast as E. coli, leading to dramatically accelerated timelines for strain optimization and scale-up.
2. What types of mutations can be screened using your HTS platform?
We can screen point mutations (via Base Editing libraries), gene repression levels (via CRISPRi libraries), and combinatorial knockouts/insertions generated by multiplex CRISPR systems.
3. How is the target product quantified in the high-throughput screening?
Quantification relies on automated methods such as plate readers for colorimetric or fluorescence-based biosensors, or specialized microplate protocols coupled with HPLC/LC-MS for chemical product analysis.
4. Can you improve the strain's ability to use a specific, low-cost feedstock?
Yes. A key area of optimization is carbon source utilization. We engineer V. natriegens to express heterologous transporters and metabolic enzymes required to efficiently metabolize non-native feedstocks like xylose or glycerol.
5. How is the stability of the engineered pathway ensured during rapid growth?
Stability is ensured by prioritizing irreversible chromosomal integration of the entire pathway, using V. natriegens's dual-chromosome system to distribute the genetic burden and prevent the loss of plasmid-borne components.
6. What is the role of computational modeling (Design) in the workflow?
Modeling predicts the effects of genetic modifications on metabolic flux and final product yield. This rational design phase focuses the lab work on the most promising targets, preventing wasted effort on suboptimal strain variants.
7. Is the final developed strain optimized for industrial fermentation conditions?
Yes. The 'Test' phase is conducted under conditions that mimic industrial fermentation (e.g., high osmotic pressure, nutrient limitations, controlled induction) to ensure the final strain is robust and performs optimally at scale.
8. What type of documentation is provided upon project completion?
We provide the final optimized strain, a detailed report including all HTS data, the complete history of genetic modifications, and performance metrics (e.g., final titer, yield, and specific productivity).
CRISPR-Cas9 technology represents a transformative advancement in gene editing techniques. The main function of the system is to precisely cut DNA sequences by combining guide RNA (gRNA) with the Cas9 protein. This technology became a mainstream genome editing tool quickly after its 2012 introduction because of its efficient, simple and low-cost nature.
The CRISPR gene editing system with its Cas9 version stands as a vital instrument for current biological research. CRISPR technology enables gene knockout (KO) through permanent gene expression blockage achieved by sequence disruption. Various scientific domains including disease modeling and drug screening employ this technology to study gene functions. CRISPR KO technology demonstrates high efficiency and precision but requires confirmation and verification post-implementation because unsatisfactory editing may produce off-target effects or incomplete gene knockouts which impact experimental result reliability. For precise and efficient Gene Editing Services - CD Biosynsis, Biosynsis offers comprehensive solutions tailored to your research needs.
The CRISPR-Cas9 knockout cell line was developed using CRISPR/Cas9 gene editing to allow scientists to remove genes accurately for research on gene function and disease models and pharmaceutical discovery. Genetic research considers this technology essential due to its high efficiency together with simple operation and broad usability.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.