CD Biosynsis offers high-speed Vibrio natriegens Protein Expression and Purification Services, leveraging the host's ultra-fast growth and high biomass capacity for rapid and scalable production of recombinant proteins. V. natriegens is a superior microbial host, boasting a doubling time of approximately 10 min, which drastically reduces the time required for fermentation and protein production compared to traditional systems like E. coli. Our comprehensive service covers everything from codon optimization and vector construction to high-cell-density fermentation and multi-step chromatography for high-purity protein isolation. We focus on optimizing the expression of challenging targets, including enzymes, therapeutic proteins, and protein subunits, ensuring clients receive highly purified, functional protein in record time for downstream applications in structural biology, drug screening, and diagnostics.
Get a QuoteThe time required for cloning, expression, and scaling up protein production is a major bottleneck in R&D. By utilizing V. natriegens, we condense fermentation time from days to hours, significantly accelerating the entire production cycle. Our platform includes proprietary expression vectors, customized media formulations for high-cell-density culture (HCDC), and tailored lysis and purification protocols to maximize both yield and purity. This rapid turnaround time, combined with robust quality control, makes V. natriegens an ideal system for projects requiring multiple protein variants or high volumes of difficult-to-express proteins.
Codon Optimization
Rational design and optimization of the target gene sequence to match the V. natriegens codon usage bias, enhancing translation efficiency and yield.
Vector Engineering
Use of specialized shuttle vectors with strong inducible promoters (e.g., arabinose, IPTG) and optimized Ribosome Binding Sites (RBS) for controlled, high-level expression.
High-Cell-Density Culture (HCDC)
Development of optimized fed-batch and continuous fermentation protocols to maximize biomass and protein concentration within the fast growth window of V. natriegens.
Affinity Chromatography (AC)
Primary capture using tags (e.g., His-tag, GST) followed by tag removal (if requested) for high-efficiency initial purification.
Ion Exchange Chromatography (IEX)
Used as a polishing step to remove residual contaminants and improve purity based on the target protein's charge properties.
Size Exclusion Chromatography (SEC)
Final polishing step used to achieve monomeric purity (>95%) and ensure structural integrity by separating based on size and molecular weight.
Metabolic & Industrial Enzymes
High-yield production of recombinant enzymes used in engineered pathways, industrial catalysis, or bioremediation applications.
Therapeutic & Vaccine Antigens
Expression of large quantities of protein fragments, antigens, or antibody domains (e.g., scFv, Fab, VHH) for drug discovery and diagnostic kits.
Difficult-to-Express Proteins
Optimization for proteins that are toxic or poorly soluble in E. coli, leveraging V. natriegens's different proteostasis and chaperone systems.
A systematic process from gene synthesis to final quality-controlled protein.
1. Design & Vector Construction
2. High-Density Expression
3. Multi-Step Purification
4. QC & Final Delivery
Codon optimize gene for V. natriegens; design purification tags (His, GST) and protease cleavage sites.
Clone gene into V. natriegens optimized shuttle vector with inducible promoter.
Transform or conjugate the vector into the host strain.
Establish small-scale culture conditions to test expression and solubility.
Scale up production using customized High-Cell-Density Culture (HCDC) media and fermentation protocols.
Induce expression at optimal cell density and harvest biomass quickly.
Purity analysis via SDS-PAGE (>95% guaranteed) and Western Blot.
Functional analysis (e.g., enzyme activity, binding kinetics) upon request.
Delivery of purified protein, QC report, and documentation of expression and purification protocols.
Unprecedented Speed
The host's 10-minute doubling time drastically shortens the fermentation phase, allowing for protein production and purification in days, not weeks, accelerating R&D timelines.
High Biomass & Productivity
V. natriegens can achieve ultra-high cell densities, directly translating to higher volumetric protein productivity and reduced costs per milligram of final protein.
E. coli Alternative
Provides a valuable alternative for proteins that are poorly expressed, insoluble, or toxic in E. coli, often resulting in higher soluble yields in V. natriegens.
Guaranteed Purity & Function
Multi-step chromatography protocols (AC, IEX, SEC) ensure delivery of protein at guaranteed purity levels (>95%), ready for demanding downstream applications.
1. How does V. natriegens compare to E. coli for protein expression speed?
V. natriegens has a doubling time of approximately 10 minutes, significantly faster than E. coli (approximately 20 minutes). This allows us to reach high-cell densities and harvest biomass in a fraction of the time, accelerating the entire protein production timeline.
2. What is the guaranteed purity level for the final protein?
We guarantee a minimum purity of 95% as verified by SDS-PAGE. Higher purity levels (e.g., >98%) can be achieved upon request through additional polishing steps like Size Exclusion Chromatography.
3. Do you provide services for non-His-tagged proteins?
Yes. While His-tag purification is standard, we also offer purification using GST, FLAG, and Strep-tag systems. For tag-free proteins, we can design custom protocols using Ion Exchange and Hydrophobic Interaction Chromatography.
4. Can you optimize the expression of a protein that is insoluble in E. coli?
Yes. V. natriegens has different chaperone and proteostasis systems than E. coli. We can optimize codon usage, fusion tags, and induction temperatures in V. natriegens, often leading to significantly higher soluble expression for traditionally difficult proteins.
5. What quality control (QC) is performed on the final protein?
Standard QC includes SDS-PAGE for purity, Western Blot for identity, and A280 measurement for concentration. Functional testing (e.g., enzyme activity, kinetic parameters) is available as an optional service.
6. Do I need to provide the expression vector?
No. We prefer to start from the gene sequence (or even just the accession number). We will perform V. natriegens-specific codon optimization and clone the gene into our proprietary expression vector optimized for this host.
7. Is the V. natriegens host genetically engineered for safety?
We typically use biosafety level 1 (BSL-1) lab strains of V. natriegens that have been engineered with modifications to ensure non-pathogenicity and auxotrophy, making them safe and suitable for industrial lab settings.
8. What is the maximum scale of protein production you offer?
We offer production ranging from small-scale expression checks (milligram quantities) up to large-scale high-cell-density fermentation (gram quantities) in bioreactors, customized to meet the client's volume requirements.
CRISPR-Cas9 technology represents a transformative advancement in gene editing techniques. The main function of the system is to precisely cut DNA sequences by combining guide RNA (gRNA) with the Cas9 protein. This technology became a mainstream genome editing tool quickly after its 2012 introduction because of its efficient, simple and low-cost nature.
The CRISPR gene editing system with its Cas9 version stands as a vital instrument for current biological research. CRISPR technology enables gene knockout (KO) through permanent gene expression blockage achieved by sequence disruption. Various scientific domains including disease modeling and drug screening employ this technology to study gene functions. CRISPR KO technology demonstrates high efficiency and precision but requires confirmation and verification post-implementation because unsatisfactory editing may produce off-target effects or incomplete gene knockouts which impact experimental result reliability. For precise and efficient Gene Editing Services - CD Biosynsis, Biosynsis offers comprehensive solutions tailored to your research needs.
The CRISPR-Cas9 knockout cell line was developed using CRISPR/Cas9 gene editing to allow scientists to remove genes accurately for research on gene function and disease models and pharmaceutical discovery. Genetic research considers this technology essential due to its high efficiency together with simple operation and broad usability.
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CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.