Pichia pastoris Protein Expression and Purification Services

Pichia pastoris is well‑suited for secreted, soluble proteins including enzymes, hormones, antibody fragments, and vaccine antigens. It can also produce membrane‑associated proteins when scoped. The system is particularly effective for proteins requiring disulfide bridges and simple N‑glycosylation.

A standard project from gene synthesis to purified protein typically takes a timeline confirmed during project scoping, depending on the complexity of the target, clone screening requirements, and scale of fermentation. Timelines are scoped during project kickoff.

We perform SDS‑PAGE, Western blot, and HPLC‑size exclusion chromatography as routine QC. Additional assays (e.g., activity, endotoxin, N‑terminal sequencing) can be included when scoped. A certificate of analysis is provided with each shipment.

Yes. We have experience scaling from shake flasks to pilot‑scale bioreactors. Large‑scale runs are scoped per project, and we provide a process description suitable for technology transfer to your facility or a CMO.

If a project involves animal studies (e.g., immunogen production for antibody generation), those activities are conducted under protocols reviewed and approved by an Institutional Animal Care and Use Committee (IACUC) or equivalent ethics oversight body. All work is performed in accordance with applicable regulations.