CD Biosynsis offers a premier, integrated platform for Phaeodactylum tricornutum Genome Editing Services, providing researchers with the most advanced tools to manipulate this model pennate diatom. As a cornerstone of marine biotechnology, Phaeodactylum tricornutum is widely recognized for its high lipid accumulation, efficient carbon fixation, and complex evolutionary background as a secondary endosymbiont. Our services are specifically engineered to navigate the unique challenges of the diatom genome, including its diploid nature and robust epigenetic silencing mechanisms. By utilizing site-specific nucleases and optimized delivery systems, we empower our clients to transform this marine microalga into a high-performance cell factory for the production of Omega-3 fatty acids, high-value pigments, and recombinant proteins.
Our expert team provides end-to-end support for diatom engineering, moving beyond traditional random mutagenesis toward rational, precision-based design. We utilize various delivery modalities—including biolistic bombardment, bacterial conjugation, and DNA-free Ribonucleoprotein (RNP) complexes—to ensure high editing efficiency across both nuclear and chloroplast genomes. Our workflow integrates sophisticated codon optimization and regulatory element selection, ensuring that every engineered strain maintains optimal growth kinetics and genetic stability. Whether you are conducting fundamental research into diatom physiology or developing industrial strains for carbon sequestration and biofuel production, our platform provides the precision and reliability needed to achieve your goals.
Get a QuoteOptimizing Phaeodactylum tricornutum requires a deep understanding of its metabolic flux and its unique intracellular architecture, including the four-membrane chloroplast. Our genome editing solutions prioritize the development of high-fidelity strains that allow for the quantitative study of marine biological processes. We employ advanced CRISPR-based systems to achieve biallelic modifications, ensuring that the desired phenotype is fully expressed in the diploid host.
Our strategic approach focuses on the rational redistribution of carbon resources. By applying systems biology principles, we identify specific genetic targets that, when modified, can redirect photosynthetic energy toward the synthesis of target metabolites such as Eicosapentaenoic acid (EPA) or fucoxanthin. This targeted strategy ensures that engineered strains achieve high specific productivity while remaining resilient under the fluctuating conditions of large-scale marine photobioreactors, bridging the gap between laboratory-scale discovery and industrial application.
Optimized CRISPR-Cas9 and Cas12a platforms utilizing codon-optimized nucleases and high-efficiency delivery systems tailored for the diatom nucleus.
Permanent biallelic disruption of target genes to eliminate metabolic competitors or study gene function in marine ecological models.
Site-specific integration of exogenous pathways or reporter tags into validated genomic safe harbors for stable, high-level expression.
Non-mutagenic, tunable transcriptional silencing for studying essential genes and balancing branched metabolic pathways.
Precise single-nucleotide conversion for targeted protein engineering and "scarless" point mutations in the diatom genome.
Our pipeline is optimized to address the specific genetic and physiological constraints of Phaeodactylum tricornutum.
1. Design & Codon Optimization
2. Tool Assembly & Transformation
3. Monoclonal Screening
4. Validation & Delivery
Selection of target loci and bioinformatic gRNA design with off-target predictive analysis. Full codon optimization of all components to match the diatom bias.
Preparation of RNP complexes or diatom-specific episomal vectors. Transformation via biolistic bombardment, conjugation, or optimized electroporation.
Verification: Genotype confirmation via Sanger or NGS sequencing. Phenotypic Analysis: Verification of target bioproduct levels and growth stability. Delivery of cryopreserved strains.
Biallelic Efficiency
Guaranteed bi-allelic modifications in the diploid host, ensuring 100% loss-of-function or stable knock-in phenotypes for immediate research use.
DNA-Free Platforms
Expertise in RNP delivery prevents foreign DNA integration, producing "cleaner" mutant backgrounds ideal for industrial regulation.
Episomal Flexibility
Utilizing replicative episomes for stable editing that can be cured post-engineering, resulting in scarless genetic modification.
Multi-Omics Validation
Comprehensive characterization of engineered strains using lipidomics and metabolic flux analysis to ensure target performance.
Technical insights for your Phaeodactylum engineering project.
Contact Us1. How do you handle the diploid nature of the Phaeodactylum tricornutum genome?
We utilize high-efficiency CRISPR-Cas9 systems designed to induce bi-allelic modifications. We confirm the success of these edits through targeted Next-Generation Sequencing (NGS) to ensure no wild-type alleles remain.
2. What is the benefit of using RNP delivery for diatoms?
RNP delivery provides transient Cas9 activity and does not involve the integration of DNA into the genome. This avoids long-term toxicity and regulatory issues associated with transgenic organisms.
3. Can you perform site-specific knock-in for multi-gene metabolic pathways?
Yes, we can integrate large multi-gene cassettes into validated genomic safe harbors using HDR-mediated repair, ensuring stable and coordinated expression of complex pathways.
4. What role does episomal delivery play in your services?
Episomal vectors allow for stable expression of editing tools without integration. They can be removed (cured) from the cell after the edit is confirmed, leaving a "clean" modified genome.
5. How is the genetic stability of the modified strains verified?
Strains undergo stability trials over 30+ passages. We verify that the genetic modifications and resulting phenotypes (e.g., lipid yield) remain constant throughout the culture period.
6. Do you provide help with codon optimization for transgenes?
Absolutely. We provide comprehensive codon optimization using proprietary Phaeodactylum-specific matrices to ensure maximal translational throughput in the host nucleus.
7. Can you perform knockouts in chloroplast genes?
Yes, we offer specialized chloroplast engineering protocols using biolistic transformation to target the plastid genome, which is essential for certain photosynthetic research projects.
8. What is the typical turnaround time for a customized diatom strain?
A standard single-gene project from design to delivery of a verified monoclonal strain typically takes 14 to 18 weeks.
CRISPR-Cas9 technology represents a transformative advancement in gene editing techniques. The main function of the system is to precisely cut DNA sequences by combining guide RNA (gRNA) with the Cas9 protein. This technology became a mainstream genome editing tool quickly after its 2012 introduction because of its efficient, simple and low-cost nature.
The CRISPR gene editing system with its Cas9 version stands as a vital instrument for current biological research. CRISPR technology enables gene knockout (KO) through permanent gene expression blockage achieved by sequence disruption. Various scientific domains including disease modeling and drug screening employ this technology to study gene functions. CRISPR KO technology demonstrates high efficiency and precision but requires confirmation and verification post-implementation because unsatisfactory editing may produce off-target effects or incomplete gene knockouts which impact experimental result reliability. For precise and efficient Gene Editing Services - CD Biosynsis, Biosynsis offers comprehensive solutions tailored to your research needs.
The CRISPR-Cas9 knockout cell line was developed using CRISPR/Cas9 gene editing to allow scientists to remove genes accurately for research on gene function and disease models and pharmaceutical discovery. Genetic research considers this technology essential due to its high efficiency together with simple operation and broad usability.
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CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.