Synthetic Biology
Human TGF beta Pathway Phosphorylation Array (8 targets)

Human TGF beta Pathway Phosphorylation Array (8 targets)

Home / Products /Assay Kits /Antibody Arrays /Phosphorylation Array /Human TGF beta Pathway Phosphorylation Array (8 targets)

Human TGF beta Pathway Phosphorylation Array (8 targets)

Catalog Number: AKAA069

Size: 2, 4, or 8

Price: Online Inquiry

Product Information
Product Name Human TGF beta Pathway Phosphorylation Array (8 targets)
Applications Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Analytes c-Jun (P-Ser73) SMAD1 (P-Ser463/465) SMAD5 (P-Ser463/465) SMAD4 (P-Thr277) ATF2 (P-Thr69/71) C-Fos (P-Thr232) SMAD2 (P-Ser245/250/255) TAK1 (P-Ser412)
ASSAY SIZE 2 Arrays/4 Arrays/8 Arrays/
Product Features Easy to useNo specialized equipment neededCompatible with nearly any liquid sampleProven technologyHighly sensitive (pg/ml)Sandwich ELISA specificityHigher density than ELISA, Western blot or bead-based multiplex
Kit Components Human TGF beta Phosphorylation Array C1 MembranesBlocking BufferDetection Antibody Cocktail500X HRP-Anti-Rabbit IgG Concentrate20X Wash Buffer I Concentrate20X Wash Buffer II Concentrate2X Cell Lysis Buffer ConcentrateDetection Buffer CDetection Buffer D8-Well Incubation Tray w/ LidProtease Inhibitor Cocktail100x Phosphatase Inhibitor Cocktail IPhosphatase Inhibitor Cocktail IIPlastic SheetsArray Map TemplateOther Materials RequiredPipettors, pipet tips and other common lab consumablesOrbital shaker or oscillating rockerTissue Paper, blotting paper or chromatography paperAdhesive tape or Saran WrapDistilled or de-ionized waterA chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline 1.Block membranes2.Incubate with Sample3.Incubate with Detection Antibody Cocktail4.Incubate with HRP-Conjugated anti-IgG5.Incubate with Detection Buffers6.Image with chemiluminescent imaging system7.Perform densitometry and analysis
Species Human
Number of Targets Detected 8
Gene Symbols ATF2, FOS, JUN, MAP3K7, SMAD1, SMAD2, SMAD4, SMAD5
Compatible Sample Types Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle Sandwich-based
Method of Detection Chemiluminescence
Pathway PKC Signaling / TGF-beta Signaling
Quantitative/Semi-Quantitative Semi-Quantitative
Storage For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Q & A

Customer Reviews

Datasheet

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Please note that all services are for research use only. Not intended for any clinical use.

Synthetic Biology Products

Online Inquiry