Product Information | |
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Product Name | Human EGFR Phosphorylation Array (16 targets) |
Applications | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
Analytes | EGFR (P-Tyr845) EGFR (P-Tyr992) EGFR (P-Tyr1045) EGFR (P-Tyr1068) EGFR (P-Tyr1086) EGFR (P-Tyr1148) EGFR (P-Tyr1173) EGFR (P-Ser1046/1047) ErbB2 (P-Tyr877) ErbB2 (P-Tyr1112) ErbB2 (P-Tyr 1221/1222) ErbB2 (P-Tyr1248) ErbB2 (P-Thr686) ErbB2 (P-Ser1113) ErbB3 (P-Tyr1289) ErbB4 (P-Tyr1284) |
ASSAY SIZE | 2 Arrays/4 Arrays/8 Arrays/ |
Product Features | Easy to useNo specialized equipment neededCompatible with nearly any liquid sampleProven technologyHighly sensitive (pg/ml)Sandwich ELISA specificityHigher density than ELISA, Western blot or bead-based multiplex |
Kit Components | Human EGFR Phosphorylation Antibody Array C1 MembranesBlocking BufferWash Buffer 1Wash Buffer 2Biotinylated Detection Antibody CocktailStreptavidin-Conjugated HRPDetection Buffer CDetection Buffer DLysis BufferProtease Inhibitor CocktailPhosphatase Inhibitor Cocktail Set IPhosphatase Inhibitor Cocktail II8-Well Incubation TrayPlastic SheetsArray TemplatesOther Materials RequiredPipettors, pipet tips and other common lab consumablesOrbital shaker or oscillating rockerTissue Paper, blotting paper or chromatography paperAdhesive tape or Saran WrapDistilled or de-ionized waterA chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system. |
Protocol Outline | 1.Block membranes2.Incubate with Sample3.Incubate with Biotinylated Detection Antibody Cocktail4.Incubate with HRP-Conjugated Streptavidin5.Incubate with Detection Buffers6.Image with chemiluminescent imaging system7.Perform densitometry and analysis |
Species | Human |
Number of Targets Detected | 16 |
Gene Symbols | EGFR, ERBB2, ERBB3, ERBB4 |
Compatible Sample Types | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
Design Principle | Sandwich-based |
Method of Detection | Chemiluminescence |
Pathway | HER/ErbB Signaling |
Quantitative/Semi-Quantitative | Semi-Quantitative |
Storage | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
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