CD Biosynsis offers specialized HeLa Cells Protein Expression and Purification Services, utilizing this robust human cell line to produce high-quality, functionally active recombinant proteins. As a human-derived eukaryotic system, HeLa cells are ideal for synthesizing complex proteins that require human-specific post-translational modifications (PTMs), native folding, and high biological activity, which cannot be achieved in bacterial or yeast hosts. Our comprehensive platform covers everything from codon optimization and expression vector construction to multi-step purification and quality control (QC). We ensure the delivery of pure, stable, and functionally validated proteins for applications in structural biology, drug screening, and antibody production.
Get a QuoteFor many biomedical applications, particularly those involving drug targets, high-resolution structural studies, or therapeutics, it is crucial that the recombinant protein possesses native human PTMs (e.g., proper glycosylation patterns and phosphorylation sites). HeLa cells provide an efficient and scalable host system that naturally supports these complex modifications, ensuring the resulting proteins exhibit correct biological activity and immunogenicity. Our service is optimized for the expression of challenging protein types, including:
We tailor our strategy based on the protein characteristics and required scale, ensuring optimal yield and purity.
Vector Construction & Optimization
Codon optimization for high expression in human cells, vector selection with strong promoters, and design of appropriate affinity tags (His-tag, Strep-tag, GST) with optional cleavage sites.
Transient Expression
Rapid, small-to-medium scale production via high-efficiency lipofection or electroporation, ideal for initial validation and quick screening.
Stable Cell Line Generation
Creation of stable, integrated HeLa clones for large-scale, consistent, and long-term high-yield protein production.
Affinity Chromatography
Primary purification step using tags (e.g., IMAC for His-tag, Glutathione for GST-tag) to rapidly isolate the target protein from the cell lysate or culture supernatant.
Ion Exchange Chromatography (IEX)
Secondary step used to remove closely co-eluting contaminants and increase the final purity percentage, based on protein charge.
Size Exclusion Chromatography (SEC)
Polishing step to ensure the protein is correctly folded and remove aggregates and oligomers, delivering a monomeric, highly active product.
Purity Assessment
Analysis via SDS-PAGE and Densitometry to determine protein molecular weight and quantify the purity percentage (typically > 95%).
Identity Confirmation
Verification using Western Blot and Mass Spectrometry (peptide mapping) to confirm the sequence and mass of the expressed protein.
Endotoxin Testing
LAL assay to ensure low endotoxin levels (typically < 1.0 EU/mg), essential for in vivo and cell-based therapeutic applications.
A controlled, stage-gated process from gene to purified protein.
1. Gene & Vector Optimization
2. Expression Strategy & Production
3. Protein Purification
4. Quality Control & Delivery
Receive target gene sequence and project specifications (scale, purity, tag).
Perform codon optimization for HeLa cell bias and synthesize the gene.
Subclone the gene into the optimized mammalian expression vector with desired tags and cleavage sites.
Select transient or stable expression strategy based on project needs.
Transfect HeLa cells at the required scale using optimized reagents (Build).
Monitor cell growth and protein expression (pilot runs) to determine optimal harvesting time.
Purity analysis via SDS-PAGE and densitometry; identity confirmation via Western Blot (Learn).
Endotoxin testing (LAL assay) and concentration determination (BCA/A280).
Package and deliver the purified protein, along with the detailed Certificate of Analysis (CoA).
Authentic Human PTMs
HeLa cells are a human host, ensuring post-translational modifications (glycosylation, phosphorylation, processing) are natively human, critical for biological activity and clinical relevance.
Complex Protein Folding
The system excels at correctly folding large, multi-domain, and complex proteins, minimizing aggregation and maximizing the yield of functionally active product.
Scalability and Consistency
HeLa cells grow robustly in suspension culture, supporting highly scalable production runs and facilitating the creation of stable cell lines for consistent long-term supply.
Low Cost Eukaryotic Alternative
Compared to other mammalian systems (like CHO or HEK293), HeLa cells offer a cost-effective platform while still maintaining essential eukaryotic features, especially for research-grade proteins.
1. Why choose HeLa cells over HEK293 or CHO cells?
HeLa cells offer an excellent balance for research-grade proteins, providing human-specific PTMs and high scalability, often at a lower cost and with faster turnaround than highly engineered commercial lines like CHO for initial expression studies.
2. What is the typical purity level of the delivered protein?
We guarantee a minimum purity of 90% for standard projects, and routinely achieve purities greater than 95% or 98% for proteins purified using three-step chromatography (Affinity, IEX, and SEC).
3. Can you produce proteins that require glycosylation?
Yes. As a eukaryotic human cell line, HeLa cells contain the necessary machinery to perform N-linked and O-linked glycosylation, ensuring the recombinant protein is modified similarly to its native human counterpart.
4. How is the endotoxin level managed?
All purification is performed using dedicated, depyrogenated materials and low-endotoxin reagents. We use the LAL assay to verify that the final product meets the standard requirement for cell-based assays (typically below 1.0 EU/mg).
5. What input materials do you require to start a project?
We typically require the full amino acid sequence (or cDNA sequence) of the target protein, specification of the desired scale (mg), required purity, and any necessary functional testing.
6. What is the difference between transient and stable expression?
Transient expression is fast and temporary, ideal for small-scale pilot runs. Stable expression involves integrating the gene into the HeLa genome, providing consistent, high-yield expression for large-scale or long-term production.
7. Can you remove the affinity tag after purification?
Yes. If the vector is designed with a specific protease cleavage site (e.g., TEV, HRV 3C) between the protein and the tag, we can perform on-column cleavage followed by a second chromatography step to remove the tag and the protease.
8. What is a Certificate of Analysis (CoA)?
The CoA is a comprehensive report provided with the final protein, including data on purity (SDS-PAGE/Densitometry), concentration (A280), verified molecular weight, and endotoxin level, ensuring full traceability and quality assurance.
CRISPR-Cas9 technology represents a transformative advancement in gene editing techniques. The main function of the system is to precisely cut DNA sequences by combining guide RNA (gRNA) with the Cas9 protein. This technology became a mainstream genome editing tool quickly after its 2012 introduction because of its efficient, simple and low-cost nature.
The CRISPR gene editing system with its Cas9 version stands as a vital instrument for current biological research. CRISPR technology enables gene knockout (KO) through permanent gene expression blockage achieved by sequence disruption. Various scientific domains including disease modeling and drug screening employ this technology to study gene functions. CRISPR KO technology demonstrates high efficiency and precision but requires confirmation and verification post-implementation because unsatisfactory editing may produce off-target effects or incomplete gene knockouts which impact experimental result reliability. For precise and efficient Gene Editing Services - CD Biosynsis, Biosynsis offers comprehensive solutions tailored to your research needs.
The CRISPR-Cas9 knockout cell line was developed using CRISPR/Cas9 gene editing to allow scientists to remove genes accurately for research on gene function and disease models and pharmaceutical discovery. Genetic research considers this technology essential due to its high efficiency together with simple operation and broad usability.
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CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.