CD Malate Dehydrogenase Assay Kit

Catalog Number: AKBA138

Size: 100 tests

(AKBA138)CD Malate Dehydrogenase Assay Kit

Product Information
Product Name	CD Malate Dehydrogenase Assay Kit
Applications	For quantitative determination of L-malate dehydrogenase enzyme activity and drug effects on malate dehydrogenase metabolism.
Features	Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 65 U/L for 20 min reaction at 37°C. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	OD565nm
Sample Type	Biological samples (e.g. plasma, serum, erythrocytes, tissue, and culture media)
Species	All
Assay Time	30 min
Limit of Detection	0.5 U/L
Expiration Date	6 months
Description	Malate dehydrogenase (MDH) (EC 1.1.1.37) is an enzyme that reversibly catalyzes the oxidation of L-malate to oxaloacetate in the presence of NAD. There are 2 isoforms in eukaryotic cells: MDH1 and MDH2. MDH1 is found in the cytoplasm and plays a key part in the malate-aspartate shuttle for transporting malate into the mitochondria. MDH2 is a mitochondrial enzyme that participates in the TCA cycle that reversibly converts L-malate into oxaloacetate. Higher MDH activities are found in some neurodegenerative diseases such as Alzheimer’s disease. BioAssay Systems’ non-radioactive, colorimetric MDH assay is based on the reduction of the tetrazolium salt MTT in an NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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CD Malate Dehydrogenase Assay Kit