Product Information | |
---|---|
Product Name | CD Farnesyltransferase Inhibitor Screening Kit |
Applications | For high-throughput screening of FTase inhibitors and evaluation of drug modulators. |
Features | Safe. Non-radioactive assay. Homogeneous and convenient. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved. High-throughput. A Z’-factor of 0.8 and higher is routinely observed in a 384-well format. Can be readily automated to assay thousands of samples per day. |
Detection Method | FL340/550 nm |
Sample Type | Compounds that affect farnesyltransferase activity |
Species | All |
Assay Time | 60 min |
Limit of Detection | NA |
Expiration Date | 6 months |
Description | FARNESYLTRANSFERASE (FTase, EC 2.5.1.58) catalyzes the transfer of a farnesyl group from farnesyl pyrophosphate to the cysteine residue of the C-terminus of target proteins. When not properly regulated, farnesylated proteins, including the Ras superfamily of small GTPases, can lead to developmental disorders and cancer. Simple, direct and high-throughput inhibitor screening assays find wide applications for oncology research. BioAssay Systems’ EIFT-400 assay kit provides a convenient fluorimetric method to screen for potential FTase inhibitors. In this assay, FTase reacts with farnesyl pyrophosphate and a dansyl-peptide substrate with measurable fluorescence at λem/ex = 340/550 nm. Inhibition is determined by the decrease in fluorescence. |
Shipping Conditions | Shipping: Ice |
Storage | Storage: -20°C upon receipt. |
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.