Biotinylation of Peptides & Small Molecules

Choose N-terminus for general labeling when orientation doesn't matter. Choose C-terminus (via Lys) or internal Lys for orientation-critical applications. Choose Cys for site-specific internal labeling when no Lys modification is desired.

No spacer works for simple detection. LC spacer is suitable for most ELISA applications. PEG spacers are recommended when maximum accessibility for streptavidin binding or reduced steric hindrance is needed.

Yes. We can conjugate biotin to small molecules (typically <2000 Da, custom scale available for larger ligands). The molecule must have an available amine or sulfhydryl group for conjugation.

Crude/Desalted is sufficient for most high-throughput screening applications. Choose >95% purity for standard research use, and >98% for diagnostic or therapeutic development where high purity is critical.