Acetylcholinesterase Reagent

Catalog Number: AKBA001

Size: 100 tests

Price: $267

Product Information
Product Name	Acetylcholinesterase Reagent
Applications	Direct assays of acetylcholinesterase activity in blood, serum, plasma, and other biological samples.
Features	Simple and Convenient. This single reagent is ready to use, when reconstituted in 25mL deionized water. High-throughput. Can be readily adapted on chemistry analyzers or high-throughput liquid handling system to test thousands of samples per day.
Detection Method	Ellman method, OD410nm
Sample Type	whole blood, serum, plasma etc
Species	All
Assay Time	10 min
Limit of Detection	6 U/L
Expiration Date	6 months
Description	ACETYLCHOLINESTERASE (EC 3.1.1.7, AChE), also known as RBC cholinesterase, is found primarily in the blood and neural synapses. Low serum cholinesterase activity may relate to exposure to insecticides or to one of a number of variant genotypes. AChE catalyzes the hydrolysis of the neurotransmitter acetylcholine into choline and acetic acid, a reaction necessary to allow a cholinergic neuron to return to its resting state after activation. Cholinesterase levels of cells and plasma are used as a guide in establishing safety precautions relative to exposure and contact, as well as a guide in determining the need for workers to be removed from areas of contact with the organic phosphate insecticides.Simple, direct and automation-ready procedures for measuring AChE activity are very desirable. BioAssay Systems’ acetylcholinesterase reagent is based on an improved Ellman method, in which thiocholine produced by the action of acetylcholinesterase forms a yellow color with 5,5′-dithiobis(2-nitrobenzoic acid). The intensity of the product color at 410 nm is proportionate to the enzyme activity in the sample.
Shipping Conditions	Shipping: RT
Storage	Storage: 2-25°C upon receipt.

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Acetylcholinesterase Reagent