Gene: ZP3
Official Full Name: zona pellucida glycoprotein 3provided by HGNC
Gene Summary: The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene. [provided by RefSeq, Jul 2008]
Catalog Number | Product Name | Species | Gene | Passage ratio | Mycoplasma testing | Price |
---|---|---|---|---|---|---|
KO02912 | ZP3 Knockout cell line (HeLa) | Human | ZP3 | 1:3~1:6 | Negative | Online Inquiry |
KO02913 | ZP3 Knockout cell line (HCT 116) | Human | ZP3 | 1:2~1:4 | Negative | Online Inquiry |
KO02914 | ZP3 Knockout cell line (HEK293) | Human | ZP3 | 1:3~1:6 | Negative | Online Inquiry |
KO02915 | ZP3 Knockout cell line (A549) | Human | ZP3 | 1:3~1:4 | Negative | Online Inquiry |
ZP3 Gene Knockout Cell Lines are genetically engineered cell lines specifically designed to lack the expression of the ZP3 gene, which encodes the zona pellucida glycoprotein 3, a vital component in mammalian fertilization. These cell lines afford researchers a unique platform for studying the roles of ZP3 in oocyte-sperm interaction, embryonic development, and reproductive physiology. By utilizing CRISPR-Cas9 or similar genome editing technologies, ZP3 knockout cell lines provide a reliable tool for dissecting the molecular mechanisms underlying fertility and the effects of disruptions in zona pellucida function.
The primary function of these cell lines is to enable the analysis of the biological effects stemming from the absence of ZP3, facilitating investigations into the cellular signaling pathways involved in gamete recognition and fertilization. Furthermore, researchers can employ these cell lines to identify potential therapeutic targets and biomarkers for infertility, thus broadening the scope of reproductive health research.
The scientific significance of ZP3 Gene Knockout Cell Lines extends to various applications in both basic and applied research. Clinically, these models are instrumental in the development of contraceptive technologies and assisted reproductive techniques, such as in vitro fertilization (IVF). By understanding the fundamental processes of fertilization, researchers can innovate strategies to enhance or inhibit conception, contributing to both fertility preservation and family planning solutions.
What sets our ZP3 Gene Knockout Cell Lines apart from alternative models is the precision and reliability of our knockout method, ensuring a complete lack of ZP3 functionality. This specificity allows for unequivocal interpretations of experimental results. Additionally, our product is backed by extensive characterization data and supports, including detailed assay protocols, which facilitate streamlined integration into existing research frameworks.
In summary, the ZP3 Gene Knockout Cell Lines are invaluable to researchers and clinicians seeking deeper insights into reproductive biology. Our company prides itself on delivering high-quality, rigorously validated biological products, supported by a team of experts dedicated to advancing the field of reproductive health research.
Please note that all services are for research use only. Not intended for any clinical use.
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