PDHX Knockout Cell Lines

Gene: PDHX

Official Full Name: pyruvate dehydrogenase complex component Xprovided by HGNC

Gene Summary: The pyruvate dehydrogenase (PDH) complex is located in the mitochondrial matrix and catalyzes the conversion of pyruvate to acetyl coenzyme A. The PDH complex thereby links glycolysis to Krebs cycle. The PDH complex contains three catalytic subunits, E1, E2, and E3, two regulatory subunits, E1 kinase and E1 phosphatase, and a non-catalytic subunit, E3 binding protein (E3BP). This gene encodes the E3 binding protein subunit; also known as component X of the pyruvate dehydrogenase complex. This protein tethers E3 dimers to the E2 core of the PDH complex. Defects in this gene are a cause of pyruvate dehydrogenase deficiency which results in neurological dysfunction and lactic acidosis in infancy and early childhood. This protein is also a minor antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC eventually leads to cirrhosis and liver failure. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2009]

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Products Background

Products

Catalog Number	Product Name	Species	Gene	Passage ratio	Mycoplasma testing	Price
KO06533	PDHX Knockout cell line (HeLa)	Human	PDHX	1:3~1:6	Negative	Online Inquiry
KO06534	PDHX Knockout cell line (HCT 116)	Human	PDHX	1:2~1:4	Negative	Online Inquiry
KO06535	PDHX Knockout cell line (HEK293)	Human	PDHX	1:3~1:6	Negative	Online Inquiry
KO06536	PDHX Knockout cell line (A549)	Human	PDHX	1:3~1:4	Negative	Online Inquiry

Background

PDHX Gene Knockout Cell Lines represent a sophisticated tool for studying the roles of the PDHX gene in cellular metabolism and disease mechanisms. The PDHX gene encodes the PDH-E3-binding protein, which is crucial in the pyruvate dehydrogenase complex responsible for energy metabolism. By employing CRISPR-Cas9 gene editing technology, these knockout cell lines facilitate the precise inactivation of the PDHX gene, allowing researchers to analyze the downstream effects on metabolic pathways and evaluate the implications on pathology, particularly in metabolic disorders and certain cancers.

The primary function of PDHX knockout cell lines is to enable the dissection of metabolic pathways by observing changes in cellular responses to nutrient availability, metabolic stress, and other external factors. The gene knockout can reveal the essential roles that PDHX plays in the regulation of glycolysis and the tricarboxylic acid cycle, consequently aiding researchers in unraveling the complexities of energy metabolism in both normal and disease states. This model system is invaluable in preclinical research, helping in the identification of potential biomarkers and therapeutic targets.

One of the significant advantages of employing PDHX Gene Knockout Cell Lines is their specificity and reproducibility compared to traditional siRNA or shRNA approaches, which can introduce off-target effects. This specificity allows for more reliable data collection and analysis, enhancing the overall research quality. Additionally, these cell lines can be easily integrated into various assays, including drug screening and metabolic profiling, making them versatile tools in both research and clinical applications.

For researchers and clinicians focused on understanding metabolic diseases or exploring novel therapeutic strategies, PDHX Gene Knockout Cell Lines offer a powerful solution, providing insights that are critical for the advancement of therapeutic interventions. Our company is committed to delivering high-quality biological products backed by extensive expertise in gene editing technologies, ensuring that our customers have access to the best tools for their research endeavors.

Please note that all services are for research use only. Not intended for any clinical use.

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