Gene: NISCH
Official Full Name: nischarinprovided by HGNC
Gene Summary: This gene encodes a nonadrenergic imidazoline-1 receptor protein that localizes to the cytosol and anchors to the inner layer of the plasma membrane. The orthologous mouse protein has been shown to influence cytoskeletal organization and cell migration by binding to alpha-5-beta-1 integrin. In humans, this protein has been shown to bind to the adapter insulin receptor substrate 4 (IRS4) to mediate translocation of alpha-5 integrin from the cell membrane to endosomes. Expression of this protein was reduced in human breast cancers while its overexpression reduced tumor growth and metastasis; possibly by limiting the expression of alpha-5 integrin. In human cardiac tissue, this gene was found to affect cell growth and death while in neural tissue it affected neuronal growth and differentiation. Alternative splicing results in multiple transcript variants encoding differerent isoforms. Some isoforms lack the expected C-terminal domains of a functional imidazoline receptor. [provided by RefSeq, Jan 2013]
Catalog Number | Product Name | Species | Gene | Passage ratio | Mycoplasma testing | Price |
---|---|---|---|---|---|---|
KO31105 | NISCH Knockout cell line (HeLa) | Human | NISCH | 1:3~1:6 | Negative | Online Inquiry |
KO31106 | NISCH Knockout cell line (HCT 116) | Human | NISCH | 1:2~1:4 | Negative | Online Inquiry |
KO31107 | NISCH Knockout cell line (HEK293) | Human | NISCH | 1:3~1:6 | Negative | Online Inquiry |
KO31108 | NISCH Knockout cell line (A549) | Human | NISCH | 1:3~1:4 | Negative | Online Inquiry |
NISCH Gene Knockout Cell Lines represent a cutting-edge advancement in genetic research, specifically designed to facilitate the study of the NISCH gene's role in various biological processes. These cell lines are prepared using state-of-the-art CRISPR-Cas9 technology, which allows for precise modifications to the genome, creating cells that lack functional copies of the NISCH gene. This targeted gene disruption enables researchers to examine the resulting phenotypic changes, thereby elucidating NISCH's involvement in pathways related to cellular growth, differentiation, and disease progression.
The key mechanism underlying the NISCH Gene Knockout Cell Lines lies in the CRISPR-Cas9 system, which introduces specific double-strand breaks at the NISCH locus. Following repair through non-homologous end joining, cells are left with mutations that abrogate the gene’s activity. This approach not only streamlines the knockout process but also significantly enhances the efficiency and reproducibility of genetic studies, making it an indispensable tool for molecular biologists.
These cell lines hold significant scientific importance in both research and clinical contexts. By providing insights into the NISCH gene's functions, they can contribute to our understanding of related diseases, such as certain cancers and developmental disorders. Furthermore, the ability to investigate gene function in controlled environments can accelerate drug discovery and the development of targeted therapies.
Compared to traditional knockout methods, such as homologous recombination, NISCH Gene Knockout Cell Lines offer unparalleled speed, simplicity, and cost-effectiveness. Researchers enjoy the benefits of ready-to-use models that can be applied in various assays, reducing the time from hypothesis to actionable data.
For researchers and clinicians, the NISCH Gene Knockout Cell Lines provide invaluable resources for elucidating the complexities of gene function and its implications for health and disease. Our company is committed to delivering high-quality biological products, supported by extensive expertise in genetic engineering and cell line development, ensuring that our offerings empower researchers in their quest to innovate and advance scientific knowledge.
Please note that all services are for research use only. Not intended for any clinical use.
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