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NEB Knockout Cell Lines

Gene: NEB

Official Full Name: nebulinprovided by HGNC

Gene Summary: This gene encodes nebulin, a giant protein component of the cytoskeletal matrix that coexists with the thick and thin filaments within the sarcomeres of skeletal muscle. In most vertebrates, nebulin accounts for 3 to 4% of the total myofibrillar protein. The encoded protein contains approximately 30-amino acid long modules that can be classified into 7 types and other repeated modules. Protein isoform sizes vary from 600 to 800 kD due to alternative splicing that is tissue-, species-,and developmental stage-specific. Of the 183 exons in the nebulin gene, at least 43 are alternatively spliced, although exons 143 and 144 are not found in the same transcript. Of the several thousand transcript variants predicted for nebulin, the RefSeq Project has decided to create three representative RefSeq records. Mutations in this gene are associated with recessive nemaline myopathy. [provided by RefSeq, Sep 2009]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO36443 NEB Knockout cell line (HCT 116) Human NEB 1:2~1:4 Negative Online Inquiry
KO36444 NEB Knockout cell line (HEK293) Human NEB 1:3~1:6 Negative Online Inquiry
KO36445 NEB Knockout cell line (A549) Human NEB 1:3~1:4 Negative Online Inquiry

Background

NEB Gene Knockout Cell Lines are genetically engineered cells that have been specifically designed to facilitate the study of gene function and regulation through targeted gene disruption. This product employs state-of-the-art CRISPR-Cas9 technology to create precise mutations in the genomic DNA of various mammalian cell lines, enabling researchers to create knockout models that are invaluable for functional genomics studies. By knocking out specific genes, scientists can investigate the biological pathways and mechanisms that underpin cell physiology and disease states.

The key functions of NEB Gene Knockout Cell Lines center around their ability to provide a reliable tool for gene editing and characterization. These cell lines exhibit defined genetic modifications that allow for robust studies in cellular responses, drug interactions, and gene-environment interactions. Researchers can utilize these knockout models to examine phenotypic changes, signaling pathways, and molecular mechanisms, ultimately leading to deeper insights into cellular behavior and disease mechanisms.

The scientific importance of these cell lines cannot be overstated, as they are essential in both research and clinical settings. In the laboratory, they serve as a cornerstone for studies related to cancer research, developmental biology, and biochemistry. In clinical environments, they hold potential for drug discovery, therapeutic development, and personalized medicine by helping to identify novel therapeutic targets and assessing the efficacy of new pharmaceutical compounds.

Compared to traditional knockout approaches, NEB Gene Knockout Cell Lines offer significant advantages including faster generation times, greater precision in gene editing, and user-friendly protocols that streamline the research process. Furthermore, these cell lines are rigorously validated and come with comprehensive documentation, ensuring that researchers can confidently replicate results and build upon their findings.

In summary, NEB Gene Knockout Cell Lines represent a transformative tool for researchers and clinicians alike, unlocking new possibilities in the pursuit of understanding complex biological systems. With extensive experience in providing high-quality biological products, our company is dedicated to supporting the scientific community with cutting-edge technologies that drive innovation and discovery.

Please note that all services are for research use only. Not intended for any clinical use.

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