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MAP3K3 Knockout Cell Lines

Gene: MAP3K3

Official Full Name: mitogen-activated protein kinase kinase kinase 3provided by HGNC

Gene Summary: This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO10686 MAP3K3 Knockout cell line (HeLa) Human MAP3K3 1:3~1:6 Negative Online Inquiry
KO10687 MAP3K3 Knockout cell line (HCT 116) Human MAP3K3 1:2~1:4 Negative Online Inquiry
KO10688 MAP3K3 Knockout cell line (HEK293) Human MAP3K3 1:3~1:6 Negative Online Inquiry
KO10689 MAP3K3 Knockout cell line (A549) Human MAP3K3 1:3~1:4 Negative Online Inquiry

Background

The MAP3K3 Gene Knockout Cell Lines are a suite of genetically engineered cell lines specifically designed to investigate the functional role of the MAP3K3 gene in various biological processes. By employing advanced CRISPR-Cas9 gene-editing techniques, these cell lines exhibit a complete knockout of the MAP3K3 gene, effectively allowing researchers to explore the gene's contributions to signal transduction pathways involved in cell proliferation, differentiation, and stress responses.

Key functions of MAP3K3 gene knockout involve the dissection of the mitogen-activated protein kinase (MAPK) signaling pathways, particularly in relation to inflammatory responses and oncogenesis. The absence of this gene provides valuable insight into the downstream signaling cascades that could be altered in various disease states, especially in cancer and autoimmune disorders. By understanding these mechanisms, scientists can pivot towards developing targeted therapies that inhibit or enhance specific pathways.

The scientific importance of the MAP3K3 Gene Knockout Cell Lines extends into both research and clinical settings. In research laboratories, these cell lines are a critical tool for the elucidation of molecular mechanisms underlying cell signaling, enabling the discovery of novel drug targets. Clinically, they pave the way for breakthroughs in personalized medicine by facilitating studies on how individual genetic variations affect treatment responses.

This product stands out in the marketplace due to its high specificity and efficiency, coupled with a user-friendly protocol that allows for straightforward application in various experimental contexts. Compared to alternative models, our MAP3K3 knockout cell lines offer consistently high knockout efficiency and reproducibility, which are paramount for reliable scientific studies.

For researchers and clinicians seeking to delve deeper into the complexities of MAP3K3-associated pathways, these cell lines provide a tangible value, empowering them to unravel critical biological processes and support translational research. Our company prides itself on its expertise in genetic engineering and cell line development, ensuring that each product is backed by rigorous quality control and extensive scientific validation. Our commitment to advancing biological research through innovative solutions positions us as a trusted partner in the scientific community.

Please note that all services are for research use only. Not intended for any clinical use.

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