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H1-5 Knockout Cell Lines

Gene: H1-5

Official Full Name: H1.5 linker histone, cluster memberprovided by HGNC

Gene Summary: Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO37428 H1-5 Knockout cell line (HEK293) Human H1-5 1:3~1:6 Negative Online Inquiry

Background

H1-5 Gene Knockout Cell Lines are specialized laboratory cell lines in which the H1-5 gene has been systematically disrupted or "knocked out" through advanced genetic engineering techniques. This product enables researchers to study the functional consequences of gene loss in a controlled setting, as these modified cell lines can serve as invaluable tools for elucidating the role of the H1-5 gene in various biological processes, including cell differentiation, proliferation, and response to external stimuli.

The primary mechanism behind the function of H1-5 Gene Knockout Cell Lines involves the use of CRISPR-Cas9 technology or other gene-editing methodologies that specifically target the H1-5 locus. By creating precise deletions or mutations within the gene sequence, these cell lines provide insights into the gene's specific contributions to cellular function or disease pathology. The loss of the H1-5 gene’s expression can subsequently lead to observable phenotypic changes, making it easier for researchers to correlate gene function and cellular behaviors.

The scientific importance of H1-5 Gene Knockout Cell Lines is multi-faceted. They are pivotal in research focused on gene function and therapeutic approaches for genetic disorders, cancer biology, and other complex diseases. In clinical settings, these cell lines can be used to screen potential drug candidates or to test the efficacy of targeted therapies, bridging the gap between laboratory findings and clinical applications.

One of the key advantages of H1-5 Gene Knockout Cell Lines over alternative models, such as traditional knockdown techniques, is the permanence of the genetic knockout, which provides a stable platform for long-term studies. This stability can yield more reproducible results, allowing for more detailed mechanistic insights. Furthermore, the use of our well-characterized cell lines minimizes variability, ensuring that experimental outcomes are both consistent and reliable.

In conclusion, H1-5 Gene Knockout Cell Lines are valuable tools for researchers and clinicians alike, facilitating in-depth investigations into gene functions and potentially leading to breakthroughs in therapeutic applications. Our company, with its expertise in cellular biology and gene editing technologies, is committed to delivering high-quality, validated biological products that empower scientists to advance their research and innovations in the life sciences.

Please note that all services are for research use only. Not intended for any clinical use.

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