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E2F5 Knockout Cell Lines

Gene: E2F5

Official Full Name: E2F transcription factor 5provided by HGNC

Gene Summary: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq, Jul 2008]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO11560 E2F5 Knockout cell line (HeLa) Human E2F5 1:3~1:6 Negative Online Inquiry
KO11561 E2F5 Knockout cell line (HCT 116) Human E2F5 1:2~1:4 Negative Online Inquiry
KO11562 E2F5 Knockout cell line (HEK293) Human E2F5 1:3~1:6 Negative Online Inquiry
KO11563 E2F5 Knockout cell line (A549) Human E2F5 1:3~1:4 Negative Online Inquiry

Background

E2F5 Gene Knockout Cell Lines are expertly engineered cellular models that lack the expression of the E2F5 transcription factor, a pivotal regulator in the cell cycle and DNA replication processes. These knockout models serve as invaluable tools for studying the downstream effects of E2F5 depletion on cellular proliferation, gene expression profiles, and the mechanisms of tumorigenesis. By removing E2F5, researchers can delineate its role in oncogenic pathways and explore alternative cell cycle regulatory mechanisms that may become activated in its absence.

The primary function of these cell lines is to facilitate in-depth investigations into the E2F family of transcription factors, which are known to be crucial not only in normal cell cycle progression but also in cancer biology. The knockout approach enables scientists to observe phenotypic alterations, changes in signaling pathways, and modifications in gene expression, providing insights that could lead to novel therapeutic targets or strategies.

In the scientific community, E2F5 Gene Knockout Cell Lines hold significant importance in both research and clinical settings. Their application extends to cancer research, gene therapy studies, and drug discovery, where understanding the dynamics of cell cycle control can pave the way for innovative treatments. Studies utilizing these cell lines can lead to breakthroughs in personalized medicine by identifying specific vulnerabilities in tumor cells linked to E2F5.

One of the unique advantages of our E2F5 knockout cell lines is their meticulous design, which ensures a high level of specificity and reproducibility across experiments. This contrasts sharply with alternative methods, such as RNA interference or pharmacological inhibition, which may yield off-target effects or varying degrees of knockdown. Researchers can rely on these cell lines for consistent results, ensuring data integrity in their studies.

For researchers, clinicians, and biotechnology companies, the E2F5 Gene Knockout Cell Lines represent a robust solution to the burgeoning demands of molecular biology studies. By utilizing these cell lines, users gain access to a reliable model for unraveling the complexities of cell cycle regulation and its implications for disease, ultimately enhancing the potential for groundbreaking discoveries.

Our company specializes in the development of high-quality biological research tools, ensuring that products like the E2F5 Gene Knockout Cell Lines are rigorously validated and supported by scientific expertise. We are committed to providing researchers with cutting-edge products that advance their work and lead to transformative results in the life sciences.

Please note that all services are for research use only. Not intended for any clinical use.

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