COL4A3 Knockout Cell Lines

Gene: COL4A3

Official Full Name: collagen type IV alpha 3 chainprovided by HGNC

Gene Summary: Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jun 2010]

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Products Background

Products

Catalog Number	Product Name	Species	Gene	Passage ratio	Mycoplasma testing	Price
KO10413	COL4A3 Knockout cell line (HeLa)	Human	COL4A3	1:3~1:6	Negative	Online Inquiry
KO10414	COL4A3 Knockout cell line (HCT 116)	Human	COL4A3	1:2~1:4	Negative	Online Inquiry
KO10415	COL4A3 Knockout cell line (HEK293)	Human	COL4A3	1:3~1:6	Negative	Online Inquiry
KO10416	COL4A3 Knockout cell line (A549)	Human	COL4A3	1:3~1:4	Negative	Online Inquiry

Background

COL4A3 Gene Knockout Cell Lines are specialized cellular models that have been genetically engineered to disrupt the COL4A3 gene, which encodes a critical component of type IV collagen. This type of collagen is essential in the formation and maintenance of basement membranes, which provide structural support to cells and tissues. The primary mechanism of action involves the use of CRISPR-Cas9 technology or similar gene-editing methods to selectively target and edit the COL4A3 gene, resulting in the absence of functional COL4A3 protein.

The significance of COL4A3 gene knockout cell lines lies in their utility for research into various pathological conditions, including Alport syndrome, a genetic disorder characterized by kidney disease, hearing loss, and eye abnormalities. Researchers can leverage these cell lines to study the molecular mechanisms underlying collagen dysregulation and to develop potential therapeutic interventions. Their application spans a wide range of fields, including nephrology, genetics, and cancer research, where understanding collagen interactions can illuminate disease mechanisms and aid in drug discovery.

Compared to traditional cell lines, which may express intact COL4A3, these knockout lines provide a clearer model for elucidating the role of this gene in vivo. Researchers benefit from higher specificity and reliability, enabling more accurate translation of findings into clinical settings. Additionally, the availability of these unique cell models allows for the exploration of therapeutic windows that target collagenopathies more effectively than current alternatives.

As experts in biological product development, we pride ourselves on delivering high-quality, rigorously validated gene knockout models that facilitate cutting-edge research. By choosing COL4A3 Gene Knockout Cell Lines from our comprehensive portfolio, researchers and clinicians are equipped with tools that enhance their ability to unlock complex biological questions and advance the state of medical science.

Please note that all services are for research use only. Not intended for any clinical use.

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