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AFF1 Knockout Cell Lines

Gene: AFF1

Official Full Name: ALF transcription elongation factor 1provided by HGNC

Gene Summary: This gene encodes a member of the AF4/ lymphoid nuclear protein related to the Fragile X E syndrome (FRAXE) family of proteins, which have been implicated in human childhood lymphoblastic leukemia, fragile chromosome X intellectual disability, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, this gene has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO04671 AFF1 Knockout cell line (HeLa) Human AFF1 1:3~1:6 Negative Online Inquiry
KO04672 AFF1 Knockout cell line (HCT 116) Human AFF1 1:2~1:4 Negative Online Inquiry
KO04673 AFF1 Knockout cell line (HEK293) Human AFF1 1:3~1:6 Negative Online Inquiry
KO04674 AFF1 Knockout cell line (A549) Human AFF1 1:3~1:4 Negative Online Inquiry

Background

AFF1 Gene Knockout Cell Lines are innovative biological products designed for advanced genetic research, specifically focusing on the gene encoding the AFF1 protein, which is implicated in various cellular processes, including transcription regulation and DNA repair mechanisms. These knockout cell lines are engineered via CRISPR-Cas9 technology to precisely disrupt the AFF1 gene, resulting in a cellular model devoid of any functional AFF1 protein.

The key function of these cell lines is to serve as a robust platform to investigate the biological role of the AFF1 protein under different physiological and pathological conditions. Researchers can utilize these knockout models to study the underlying mechanisms of gene expression, cellular stress responses, and their association with diseases such as leukemia and other cancers where AFF1 may play a critical role. The AFF1 gene knockout cell lines facilitate a deeper understanding of the consequences of gene loss, extending their utility in functional genomics, drug discovery, and therapeutic target validation.

The scientific importance of these cell lines lies not only in their applications for basic research but also in their potential to advance translational medicine. These models allow clinicians and researchers to explore how the absence of specific gene functions influences tumor progression or drug sensitivity, offering insights that can lead to personalized treatment strategies.

One major advantage of our AFF1 Gene Knockout Cell Lines over traditional models is their high fidelity and reproducibility, ensuring that experimental results are consistent and reliable. Unlike other methods that utilize broad-spectrum gene silencing techniques, the precise gene knockout guarantees that the effects observed are directly attributable to the gene of interest, eliminating confounding variables.

For researchers and clinicians, the value of these cell lines cannot be overstated; they offer an unparalleled resource for elucidating gene function and pathology, empowering users to make breakthroughs in their research. Our company is committed to providing cutting-edge biological products and has a proven track record in the development of high-quality genetic models. With our expertise in molecular biology and a focus on innovation, we aim to support the scientific community with tools that propel medical and biological discoveries forward.

Please note that all services are for research use only. Not intended for any clinical use.

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