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ADD3 Knockout Cell Lines

Gene: ADD3

Official Full Name: adducin 3provided by HGNC

Gene Summary: Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known. [provided by RefSeq, Jul 2008]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO03877 ADD3 Knockout cell line (HeLa) Human ADD3 1:3~1:6 Negative Online Inquiry
KO03878 ADD3 Knockout cell line (HCT 116) Human ADD3 1:2~1:4 Negative Online Inquiry
KO03879 ADD3 Knockout cell line (HEK293) Human ADD3 1:3~1:6 Negative Online Inquiry
KO03880 ADD3 Knockout cell line (A549) Human ADD3 1:3~1:4 Negative Online Inquiry

Background

ADD3 Gene Knockout Cell Lines are precisely engineered cell models designed to eliminate the expression of the ADD3 gene, which encodes a significant cytoskeletal protein involved in cellular signaling and structural integrity. These knockout cell lines facilitate in-depth functional studies, enabling researchers to explore the biological pathways and mechanisms impacted by the absence of ADD3. The deliberate disruption of the ADD3 gene provides a powerful tool for examining its role in various cellular processes, including morphogenesis, migration, and response to oxidative stress.

The key function of ADD3 Gene Knockout Cell Lines lies in their ability to mimic disease states related to ADD3 dysfunction, offering a platform for both academic and pharmaceutical research. By employing CRISPR/Cas9 gene editing technology, these cell lines ensure a precise and reproducible knockout, allowing for consistent experimental results. Researchers can study the downstream effects of deleting ADD3, investigate compensatory mechanisms, or screen potential therapeutic compounds aimed at restoring normal cellular function.

From a scientific perspective, ADD3 Gene Knockout Cell Lines are invaluable in advancing our understanding of diseases such as cancer, neurodegenerative disorders, and developmental abnormalities. They provide insights into potential pathways for targeted therapies, which can be translated from bench to bedside. Compared to alternative models, these cell lines offer more accurate depictions of pathological states because they maintain the native cellular environment alongside the absence of the gene of interest.

For researchers and clinicians, the value of ADD3 Gene Knockout Cell Lines is underscored by their versatility and reliability. They empower the scientific community with tools necessary to dissect complex biological questions and develop innovative treatment strategies. Supporting this offering is our company’s extensive expertise in cell line development and gene editing technologies, which guarantees the highest quality products tailored for scientific advancement.

Please note that all services are for research use only. Not intended for any clinical use.

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