Target-Class Specific Profiling Services for Drug Discovery

CD Biosynsis offers comprehensive Target-Class Specific Profiling services, providing focused, high-quality assay panels for the most critical enzyme families and receptor systems in modern drug discovery. Our platform is designed to move beyond single-target screening, offering customized panels that assess compound potency, selectivity, and mechanism of action (MoA) across entire target classes, including kinases, proteases, GPCRs, and nuclear receptors. By focusing on target families, we enable researchers to rapidly identify selective leads, minimize off-target toxicity, and accelerate preclinical validation. Utilize our established, validated assay platforms—from high-throughput functional assays to detailed kinetic studies—to build a precise profiling strategy tailored to your therapeutic area.

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Focused and Mechanistic Profiling Across Key Drug Targets

Selecting the right target class for detailed profiling is a critical decision in lead optimization. Our target-class specific services ensure that your compounds are tested against a relevant panel of homologous targets to establish a robust selectivity profile. This is crucial for target families like Kinases, where off-target inhibition can lead to serious safety liabilities, or GPCRs, where signaling bias can define therapeutic outcome. We provide standardized workflows for each major class, ensuring reproducible, high-quality data. Whether you are characterizing small molecule inhibitors, PROTACs, or antibodies, our specialized platforms deliver the mechanistic clarity required to confidently advance your drug candidates.

Available Target-Class Profiling Modules

Key Enzyme Classes Receptor and Signaling Classes Complex Pathway Profiling

Specialized Enzyme Family Profiling

Services focused on major enzyme targets for drug discovery:

Kinase and Phosphatase Profiling Services

Comprehensive selectivity and kinetic analysis across the entire human kinome and major phosphatase families.

Protease and Peptidase Profiling Services

Functional assays for screening inhibitors against Cysteine, Serine, Aspartic, and Metalloproteases.

ATPase and GTPase Profiling Services

Assays for measuring nucleotide hydrolysis and inhibition of essential cellular motor proteins and signaling regulators (e.g., Ras superfamily).

Poly(ADP-Ribose) Polymerase (PARP) Assays and Profiling

Dedicated assays for characterizing PARP inhibitors in DNA damage response and oncology research.

Phosphodiesterase (PDE) Assays and Profiling

Selectivity profiling across all 11 PDE families (PDE1-PDE11) for cyclic nucleotide signaling modulators.

Key Receptor and Signaling Classes

Services focused on profiling membrane and nuclear receptors:

Ion Channel Assays and Functional Profiling

Electrophysiology and flux assays for characterizing voltage-gated, ligand-gated, and TRP channels (including hERG safety).

GPCR Assays and Functional Signaling Profiling

Multiparametric assays for Gs, Gi, Gq coupling, arrestin recruitment, and biased agonism analysis.

Nuclear Receptor (NR) Assays and Functional Profiling

Binding, co-regulator recruitment, and RGA assays for Steroid, Metabolic, and Orphan NRs.

Complex Pathway and Degradation Profiling

Services targeting integrated regulatory systems:

Targeted Protein Degradation (TPD) Assays and Profiling

Functional assays for characterizing PROTACs, including ternary complex formation, ubiquitination, and degradation kinetics.

Ubiquitin-Proteasome System (UPS) Assays and Profiling

Profiling E1, E2, E3 Ligases, DUBs, and 20S/26S Proteasome activity for oncology and neurodegeneration.

Apoptosis Pathway Assays and Profiling Services

Cell-based and biochemical assays for key components, including Caspases and Bcl-2 family proteins.

Metabolic Pathway Assays and Functional Profiling

Characterization of key enzymes in glycolysis, fatty acid oxidation, and other central metabolic pathways.

General Target-Class Profiling Workflow

A standardized approach ensuring high quality and relevance for all target classes.

Panel Definition and Reagent Sourcing

Assay Platform Optimization

Compound Screening and Potency Determination

Mechanistic Analysis and Data Reporting

Target Selection: Based on client needs, a custom panel of related targets (e.g., 50 Kinases, 10 GPCRs) is defined.

Reagent QC: All recombinant enzymes, cell lines, and substrates specific to the target class are validated for activity and purity.

HTS Setup: Implement the most suitable high-throughput technology (e.g., TR-FRET for kinases, FLIPR for GPCRs, RGA for NRs).

Optimization: Conditions are fine-tuned to ensure linearity, stability, and high statistical robustness (Z-factor > 0.7).

Potency Screening: Perform full 10-point dose-response curves for test compounds against the entire custom panel.

Selectivity Index: Calculate the ratio of IC50/EC50 values to determine compound specificity against off-targets.

Advanced Kinetics: Perform Km/Vmax/Ki determination or functional bias analysis (GPCRs) for lead compounds.
MoA Confirmation: Use specific class-appropriate assays (e.g., ATP competition for Kinases, Gating for Ion Channels) to confirm mechanism.
Reporting: Deliver comprehensive final reports with raw data, analyzed plots, and clear interpretation of selectivity.

The CD Biosynsis Advantage in Target Profiling

Integrated Target Libraries

Immediate access to validated recombinant enzymes and cell lines covering the full spectrum of druggable targets.

Custom Selectivity Panels

Build customized panels for any combination of targets (e.g., Kinases + GPCRs) based on therapeutic area risk.

Mechanistic Clarity

Services extend beyond IC50 to MoA, including allosteric site analysis, signaling bias, and degradation kinetics.

Reduced Off-Target Risk

Detailed selectivity data accelerates lead optimization by filtering out compounds with high predicted toxicity.

Client Testimonials on Target-Class Profiling

"The Kinase selectivity panel was run flawlessly. The data clearly showed the minimal off-target activity, giving us the confidence to move our lead into in vivo studies."

Dr. Alex Thompson, Oncology R&D Director

"Using the GPCR biased agonism module, we quickly characterized our novel analgesic's unique signaling profile, which is key to avoiding respiratory depression."

Ms. Isabella Rossi, Neuroscience Research Lead

"The UPS profiling services allowed us to identify the E3 Ligase specificity of our PROTAC, drastically reducing the time spent on mechanistic validation."

Mr. Kai Zhang, Chemical Biology Manager

FAQs about Target-Class Specific Profiling

Still have questions?

What is the main benefit of Target-Class Specific Profiling over single-target screening?

It provides crucial selectivity data early in the discovery process, comparing your lead compound against a panel of closely related off-targets. This minimizes the risk of toxicity and side effects due to off-target inhibition.

Which assay methods are primarily used for Kinase profiling?

Kinase profiling typically uses homogeneous assays such as TR-FRET, AlphaScreen, or mobility shift assays, which allow for high-throughput screening of ATP and substrate competition.

Can you perform TPD assays for E3 ligases not in your standard panel?

Yes. We offer custom assay development for novel or less common E3 ligases, including setting up assays for ternary complex formation and measuring target protein degradation kinetics via Western Blot or high-content imaging.

Is hERG screening included in the Ion Channel profiling service?

Yes, hERG (Kv11.1) is a critical component of our Ion Channel safety panel. We perform high-fidelity hERG screening using Automated Patch Clamp (APC) to ensure compliance with preclinical safety assessment standards.

How much does Metabolic Engineering services cost?

The cost of Metabolic Engineering services depends on the project scope, complexity of the target compound, the host organism chosen, and the required yield optimization. We provide customized quotes after a detailed discussion of your specific research objectives.

Do your engineered strains meet regulatory standards?

We adhere to high quality control standards in all strain construction and optimization processes. While we do not handle final regulatory approval, our detailed documentation and compliance with best laboratory practices ensure your engineered strains are prepared for necessary regulatory filings (e.g., GRAS, FDA).

What to look for when selecting the best gene editing service?

We provide various gene editing services such as CRISPR-sgRNA library generation, stable transformation cell line generation, gene knockout cell line generation, and gene point mutation cell line generation. Users are free to select the type of service that suits their research.

Does gene editing allow customisability?

Yes, we offer very customised gene editing solutions such as AAV vector capsid directed evolution, mRNA vector gene delivery, library creation, promoter evolution and screening, etc.

What is the process for keeping data private and confidential?

We adhere to the data privacy policy completely, and all customer data and experimental data are kept confidential.