Kinase and Phosphatase Profiling Services

CD Biosynsis offers specialized Kinase and Phosphatase Profiling Services, essential for drug discovery programs targeting these crucial regulatory enzymes. These enzymes are key players in cellular signaling pathways, making them prime targets for therapeutic intervention, particularly in oncology and immunology. Our services provide high-quality, quantitative characterization of enzyme activity and inhibitor potency (IC50, Ki). Utilizing advanced technologies like Caliper mobility shift assays, radiometric detection, and highly sensitive fluorescence polarization, we ensure robust, reliable, and reproducible results critical for lead optimization and mechanism of action studies. Choose from our expansive library of validated human kinases and phosphatases to build a custom profiling panel tailored to your project needs.

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Precision Characterization for Signaling Pathways

Kinases (transferring phosphate groups) and Phosphatases (removing phosphate groups) maintain the critical balance of cellular phosphorylation, a pervasive regulatory mechanism. Accurate profiling is vital not only for identifying potent drug candidates but also for assessing off-target effects and selectivity, minimizing toxicity and increasing therapeutic window. Our service leverages a comprehensive library of validated human enzymes, coupled with specialized substrates and state-of-the-art detection instrumentation. We offer flexibility, from single-target kinetic analysis to large-scale, multiplexed inhibitor screening across entire enzyme families. By providing detailed insight into inhibitor mechanism, we empower researchers to make rapid, data-driven decisions during preclinical development.

Kinase and Phosphatase Profiling Workflow

A rigorous process from target validation to hit characterization.

Target Panel Selection and Sourcing

Assay Platform Selection and Optimization

Screening and Potency Confirmation

Advanced Kinetics and Reporting

Panel Customization: Finalize the list of Kinase and Phosphatase targets based on client selection and therapeutic focus.

Enzyme Quality Control: Verify purity (> 90 percent) and activity of all recombinant enzymes (in-house or client-supplied).

Technology Selection: Choose the optimal detection method (e.g., Caliper EZ Reader, HTRF, Fluorescence) for each selected enzyme.

Assay Optimization: Titrate enzyme, substrate, and ATP/phosphate concentrations to ensure robust, linear kinetics and high Z-factor (> 0.7).

HTS Execution: Perform initial screening or IC50 dose-response curves for all test compounds against the customized panel.

Potency Confirmation: Run detailed dose-response curves for all confirmed hits to determine inhibitory strength (IC50).

Mechanistic Analysis: Perform full kinetic studies at varying substrate/ATP concentrations to determine the Ki and inhibition mode.
Selectivity Index: Calculate the specificity ratios based on the IC50 values across the entire custom panel.
Reporting: Deliver raw data, analyzed plots, IC50/Ki values, and full technical protocols.

Expertise in Regulatory Enzyme Profiling

Customizable Enzyme Panel

Freedom to select and combine any Kinase or Phosphatase from our library of over 300 targets for focused profiling.

Diverse Assay Technologies

Access to state-of-the-art platforms (Caliper, HTRF, FP, Radioactivity) for optimal data quality across all enzyme types.

Mechanistic Detail (Ki)

Precise determination of Ki and inhibition mode, distinguishing between ATP-competitive and allosteric inhibitors.

Rapid HTS Capability

High-throughput screening platforms capable of rapidly processing large inhibitor libraries with exceptional data quality.

Client Testimonials on Kinase and Phosphatase Profiling

"The detailed Ki determination provided crucial proof of mechanism for our lead compound, confirming its non-ATP competitive mode against our target kinase."

Dr. Samuel Liu, Medicinal Chemistry Director

"Their selectivity profiling against the 300-kinase panel allowed us to confidently proceed with our candidate, showing exceptional specificity against closely related targets."

Ms. Anya Sharma, Preclinical Development Lead

"The phosphatase substrate mapping identified the precise phosphopeptide motif favored by our target, guiding the rational design of next-generation inhibitors."

Mr. Julian Chen, Molecular Biology Researcher

FAQs about Kinase and Phosphatase Profiling

Still have questions?

What is the most common method for Kinase inhibitor screening?

The Caliper mobility shift assay is highly common, alongside fluorescence polarization (FP) and HTRF, due to their high Z-factors, low reagent consumption, and compatibility with HTS automation.

How do you determine the mechanism of inhibition (Ki)?

The inhibition constant (Ki) and mechanism are determined by measuring the IC50 value at varying, fixed concentrations of one substrate (e.g., ATP) while keeping the other substrate constant, followed by advanced kinetic modeling.

Can you perform profiling on membrane-bound or complex kinases?

Yes, we have specialized protocols and reagents to handle challenging targets, including membrane-associated kinases and complex multi-subunit phosphatases, ensuring they remain active and stable during the assay.

What sample purity is required for profiling?

We typically require the enzyme sample (kinase or phosphatase) to have a minimum purity of 85-90 percent by SDS-PAGE to ensure accurate and reproducible kinetic measurements without interference from contaminating proteins.

How much does Metabolic Engineering services cost?

The cost of Metabolic Engineering services depends on the project scope, complexity of the target compound, the host organism chosen, and the required yield optimization. We provide customized quotes after a detailed discussion of your specific research objectives.

Do your engineered strains meet regulatory standards?

We adhere to high quality control standards in all strain construction and optimization processes. While we do not handle final regulatory approval, our detailed documentation and compliance with best laboratory practices ensure your engineered strains are prepared for necessary regulatory filings (e.g., GRAS, FDA).

What to look for when selecting the best gene editing service?

We provide various gene editing services such as CRISPR-sgRNA library generation, stable transformation cell line generation, gene knockout cell line generation, and gene point mutation cell line generation. Users are free to select the type of service that suits their research.

Does gene editing allow customisability?

Yes, we offer very customised gene editing solutions such as AAV vector capsid directed evolution, mRNA vector gene delivery, library creation, promoter evolution and screening, etc.

What is the process for keeping data private and confidential?

We adhere to the data privacy policy completely, and all customer data and experimental data are kept confidential.

Kinase and Phosphatase Profiling Services

Precision Characterization for Signaling Pathways

Kinase and Phosphatase Target Profiling Options

Selectable Kinase Panel (Examples)

Choose Kinase Family Members for Profiling

Selectable Phosphatase Panel (Examples)

Choose Phosphatase Family Members for Profiling

Available Profiling Methods for Selected Targets

Assay Types and Kinetic Services

Kinase and Phosphatase Profiling Workflow

Expertise in Regulatory Enzyme Profiling

Client Testimonials on Kinase and Phosphatase Profiling

FAQs about Kinase and Phosphatase Profiling