Enzyme Fermentation Development Services

CD Biosynsis provides specialized Enzyme Fermentation Development services to bridge the gap between lab-scale expression and industrial production. Our expertise lies in optimizing microbial and yeast host systems (E. coli, Pichia, Saccharomyces) to maximize target enzyme yield and purity while minimizing costs and production time. We cover the entire bioprocess pipeline, from media formulation and strain optimization to upstream process development (fermentation) and preliminary downstream recovery planning. Our platform utilizes advanced bioreactor technology and process control strategies to ensure high volumetric productivity and consistent batch quality, making your enzyme ready for large-scale commercial, diagnostic, or therapeutic manufacturing.

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Optimizing Bioprocesses for Industrial Enzyme Production

Efficient large-scale enzyme production hinges on robust fermentation protocols. Unlike small-scale expression, fermentation development focuses on managing parameters such as dissolved oxygen, pH, temperature, and feeding strategies within controlled bioreactors to sustain high cell density and maximize product formation. Our service includes strain stability testing, media component screening (chemically defined or complex), and implementing strategies like fed-batch or continuous fermentation. This rigorous optimization process ensures that the enzyme yield is not only high but also reproducible and scalable, translating seamlessly from 1-liter bench scale to 100-liter or larger pilot scales.

Customizable Fermentation Development Modules

Strain & Medium Optimization Bioreactor Process Development Scalability and Downstream Planning

Strain and Medium Optimization Services

Select modules to enhance strain performance and production yield:

Check the box next to the optimization step you wish to include in your fermentation project:

Host Strain Stability Assessment

Media Component Screening (DoE)

Chemically Defined Medium Setup

Codon Optimization for High Yield

Promoter/Inducer System Optimization

Plasmid Copy Number Control

Metabolic Flux Analysis

Toxin/Protease Minimization

Bioreactor Process Development

Select the specialized fermentation strategies for controlled, high-density growth:

Select the bioprocess modules required for optimizing production:

Batch Fermentation

Fed-Batch Fermentation Optimization

Continuous Fermentation (Chemostat)

High Cell Density Culture (HCDC)

Dissolved Oxygen (DO) Control Strategy

pH and Temperature Profile Optimization

Cell Disruption / Harvest Optimization

Excretion Pathway Optimization

Scalability, Transfer, and Documentation

Ensuring the developed process is ready for scale-up and regulatory submission:

Scale-Up Feasibility Study

Testing optimized parameters at intermediate scale (e.g., 5L to 50L) to confirm reproducibility.

Preliminary DSP Planning

Initial assessment and optimization of primary recovery steps (centrifugation, filtration, cell lysis).

Full Process Documentation

Detailed Standard Operating Procedures (SOPs) and batch records suitable for technology transfer.

Fermentation Process Development Workflow

A staged process for optimizing yield and scalability.

Bench Scale Feasibility & Media Screening

Bioreactor Process Optimization (Upstream)

Process Lock & Preliminary DSP

Scale-Up and Technology Transfer

Strain Validation: Confirm stability and expression in flasks/shake tubes.

Medium Screening: Test various media compositions (complex vs. defined) to maximize biomass and target protein titer.

Bioreactor Control: Establish control loop parameters (pH, DO, temperature) in 1-5L reactors.

Feeding Strategy: Optimize fed-batch regimen (e.g., carbon source rate) to prevent substrate inhibition and maximize product yield.

Process Lock: Finalize the master batch record and perform confirmatory runs with fixed parameters.

Primary Recovery: Optimize harvest and lysis methods to maximize enzyme release and stability prior to purification.

  • Pilot Runs: Conduct scale-up runs (e.g., 50L) to confirm process fidelity and yield reproducibility at a larger scale.
  • Documentation: Generate a comprehensive process transfer package including SOPs and raw data.
  • Delivery: Provide optimized strain, frozen cell paste, and documentation for client manufacturing.

From Bench to Bioreactor: Maximizing Yield and Consistency

High Volumetric Productivity

           

Focus on maximizing enzyme yield per liter of culture volume through high cell density techniques.

Scalability Assurance

           

Process developed and optimized for seamless, predictable scale-up from lab bench to commercial bioreactor sizes.

Cost-Efficiency

           

Reduction of production costs through optimized media formulation and simplified downstream recovery planning.

Robust Documentation

           

Provision of GMP-ready documentation for straightforward regulatory and technology transfer purposes.

Client Testimonials on Enzyme Fermentation Development

"The fed-batch optimization for our therapeutic enzyme in E. coli increased the final titer by 400%, which was a game-changer for our manufacturing cost model."

Dr. Samuel Rivera, Bioprocess Engineering Lead

"Their team successfully transferred our Pichia expression system to a fully defined media, which simplified downstream processing and met our regulatory requirements."

Ms. Anya Petrova, Manufacturing Science Manager

"The detailed process documentation they provided made the scale-up from 10L to 100L at our CDMO partner completely seamless and predictable."

Mr. Kenzo Tanaka, Project Management Director

FAQs about Enzyme Fermentation Development

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What is the difference between batch and fed-batch fermentation?

Batch fermentation is a closed system where all nutrients are added at the start. Fed-batch fermentation involves feeding nutrients (often the carbon source) incrementally during the process, which allows for higher cell densities and prevents substrate inhibition, leading to higher product yield.

Why is strain stability assessment important?

In large-scale production, prolonged growth can lead to genetic drift or plasmid loss in microbial strains. Stability assessment ensures that the production strain maintains its high-yielding phenotype throughout the entire fermentation run, guaranteeing consistent batch quality.

Can you develop fermentation processes for yeast systems?

Yes. We have specialized expertise in yeast systems like Pichia pastoris and Saccharomyces cerevisiae, often utilized for producing secreted proteins that require complex post-translational modifications (PTMs).

What scale of fermentation development do you offer?

Our development and optimization work is typically conducted at the 1-10L scale. We then conduct scale-up feasibility runs up to 50-100L to validate the process before transferring the finalized SOPs for larger commercial manufacturing.

How much does Metabolic Engineering services cost?

The cost of Metabolic Engineering services depends on the project scope, complexity of the target compound, the host organism chosen, and the required yield optimization. We provide customized quotes after a detailed discussion of your specific research objectives.

Do your engineered strains meet regulatory standards?

We adhere to high quality control standards in all strain construction and optimization processes. While we do not handle final regulatory approval, our detailed documentation and compliance with best laboratory practices ensure your engineered strains are prepared for necessary regulatory filings (e.g., GRAS, FDA).

What to look for when selecting the best gene editing service?

We provide various gene editing services such as CRISPR-sgRNA library generation, stable transformation cell line generation, gene knockout cell line generation, and gene point mutation cell line generation. Users are free to select the type of service that suits their research.

Does gene editing allow customisability?

Yes, we offer very customised gene editing solutions such as AAV vector capsid directed evolution, mRNA vector gene delivery, library creation, promoter evolution and screening, etc.

What is the process for keeping data private and confidential?

We adhere to the data privacy policy completely, and all customer data and experimental data are kept confidential.

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