Custom Enzyme Production Services

CD Biosynsis offers comprehensive Custom Enzyme Production services, providing highly pure and functionally active recombinant enzymes tailored for research, diagnostics, and industrial applications. Our integrated platform covers the entire workflow, starting from gene synthesis and expression host selection to final large-scale fermentation and purification. We specialize in overcoming challenges associated with complex targets, including those requiring specific post-translational modifications (PTMs), membrane insertion, or high yields for industrial scale-up. Researchers can choose between our detailed small-scale purification services for initial characterization or leverage our full fermentation development pipeline for commercial quantity production, ensuring consistency and quality at every stage.

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Integrated Solutions for Functional Enzyme Supply

Custom Enzyme Production requires optimization at both the molecular level (expression system, construct design) and the process level (fermentation parameters, purification methods). We manage this complexity by offering a modular approach. Our team selects the most appropriate expression host (bacterial, yeast, insect, mammalian) to ensure correct protein folding and activity, followed by a purification strategy optimized for yield and purity (up to 98%). For clients needing large volumes, our fermentation development expertise ensures that the process is robust, scalable, and cost-effective, ready for smooth technology transfer to manufacturing partners. This ensures a reliable supply of high-quality enzyme crucial for therapeutic, diagnostic, or biocatalysis applications.

Available Custom Enzyme Production Modules

Microgram to Gram Scale Production Bioprocess Scale-Up Development Challenging Targets & QC

Module 1: Enzyme Expression and Purification

Services focused on producing high-purity enzyme for assay development and structural studies:

Enzyme Expression and Purification Services

Detailed optimization of expression hosts (E. coli, Yeast, Insect, Mammalian) and multi-step chromatography for milligram-scale, high-purity enzyme delivery (up to 98%).

Module 2: Enzyme Fermentation Development

Services focused on optimizing production processes for industrial and commercial scale-up:

Enzyme Fermentation Development Services

Upstream bioprocess optimization, including fed-batch strategies, media screening, and scale-up feasibility studies, to achieve high volumetric productivity for manufacturing.

Specialized Enzyme Production Capabilities

Expertise in handling complex and difficult-to-express proteins:

Multi-Subunit Complex Assembly

Co-expression and purification of protein complexes and multi-enzyme systems in a functional state.

Post-Translational Modification (PTM) Management

Strategic use of host systems (e.g., mammalian) to ensure correct glycosylation, phosphorylation, or lipidation.

Rigorous Quality Control

Activity testing (U/mg), Endotoxin testing, and detailed mass spectrometry analysis on all final enzyme products.

Integrated Custom Enzyme Production Workflow

A seamless process spanning gene design to final delivery of functional enzyme.

Gene & Construct Design

Pilot Expression & Purification

Fermentation Development (Scale-Up)

Final QC, Delivery, & Documentation

Codon Optimization: Design gene for optimal expression in the chosen host system.

Vector Selection: Cloning into expression vectors compatible with small-scale or large-scale production (e.g., pET, Baculo, CHO).

Host Screening: Test 2-3 hosts/conditions to identify the highest yield and solubility pathway.

Initial Purification: Perform multi-step chromatography to deliver an initial high-purity, active sample for functional validation.

Process Optimization: If large scale is required, optimize fermentation parameters (media, DO, feed strategy) in bioreactors.

Pilot Run: Validate the robust, scalable fermentation process at an intermediate volume (e.g., 50L) for yield confirmation.

Activity Assurance: Confirm functional integrity (Specific Activity, Kd) and high purity (SDS-PAGE >95%).
Low Endotoxin: Optional testing and removal for cell-based and in vivo applications.
Documentation: Provide Certificate of Analysis (CoA), SOPs, and full process transfer package.

Precision Engineering for Reliable Enzyme Supply

Integrated Workflow

Seamless transition from gene design and milligram-scale purification to large-scale fermentation.

Maximum Activity & Purity

Guaranteed high purity (up to 98%) combined with mandatory functional activity verification.

Cost-Effective Scale-Up

Fermentation optimization reduces production costs and ensures robust, reproducible yield for commercial needs.

Handling Complexity

Expertise in difficult targets: membrane proteins, multi-enzyme complexes, and PTM-dependent enzymes.

Client Testimonials on Custom Enzyme Production

"The integrated service from purification optimization to 50L fermentation development was critical. It saved us significant time and capital expenditure."

Dr. Ricardo Gomez, Biologics Manufacturing Director

"We struggled to get soluble expression of our target enzyme. Their team successfully moved it to a Pichia system, delivering functional protein well ahead of schedule."

Ms. Janet Foster, Assay Development Lead

"The purity and activity of the delivered enzyme were consistently excellent across all batches, making our HTS campaign highly reliable."

Mr. Hiroshi Sato, Research Operations Manager

FAQs about Custom Enzyme Production

Still have questions?

What determines the choice between E. coli and mammalian expression?

The main determinant is the need for Post-Translational Modifications (PTMs). E. coli is faster and cheaper but cannot perform complex PTMs like glycosylation. Mammalian systems (CHO, HEK) are used for enzymes requiring complex PTMs or high secretion yields.

What level of purity can you guarantee?

We typically guarantee a purity level of >90%, with the option to achieve >95% or >98% purity for highly demanding applications like structural studies or specialized kinetic assays, confirmed by SDS-PAGE and densitometry.

Can you develop the fermentation process for my existing cell line?

Yes. If you provide a validated, high-expressing strain (e.g., E. coli clone, Pichia culture), we can take that strain directly into our fermentation development pipeline for process optimization and scale-up feasibility studies.

Is Endotoxin testing part of the standard QC?

Purity and activity checks are standard. Endotoxin testing and removal (for Low Endotoxin grade) is offered as an essential optional QC module required for any enzyme intended for cell culture or in vivo work.

How much does Metabolic Engineering services cost?

The cost of Metabolic Engineering services depends on the project scope, complexity of the target compound, the host organism chosen, and the required yield optimization. We provide customized quotes after a detailed discussion of your specific research objectives.

Do your engineered strains meet regulatory standards?

We adhere to high quality control standards in all strain construction and optimization processes. While we do not handle final regulatory approval, our detailed documentation and compliance with best laboratory practices ensure your engineered strains are prepared for necessary regulatory filings (e.g., GRAS, FDA).

What to look for when selecting the best gene editing service?

We provide various gene editing services such as CRISPR-sgRNA library generation, stable transformation cell line generation, gene knockout cell line generation, and gene point mutation cell line generation. Users are free to select the type of service that suits their research.

Does gene editing allow customisability?

Yes, we offer very customised gene editing solutions such as AAV vector capsid directed evolution, mRNA vector gene delivery, library creation, promoter evolution and screening, etc.

What is the process for keeping data private and confidential?

We adhere to the data privacy policy completely, and all customer data and experimental data are kept confidential.