Enzyme Expression and Purification Services

CD Biosynsis offers expert, customized Enzyme Expression and Purification services crucial for assay development, structural biology, and HTS campaigns. The quality and activity of your target enzyme are paramount to the success of any drug discovery project. We specialize in producing high-purity, highly active recombinant enzymes, ranging from soluble cytoplasmic proteins to complex membrane-associated enzymes and multi-subunit complexes. Our platform allows for optimization across various expression systems, including bacterial (E. coli), yeast, insect, and mammalian cells, ensuring optimal folding and post-translational modifications. Researchers can select specific expression hosts, purification tags, and quality control metrics to receive a product perfectly suited for their downstream functional or structural studies.

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Customized Production of High-Quality, Active Recombinant Enzymes

Producing functional enzymes, especially those with complex structures or requiring specific post-translational modifications (PTMs), presents significant challenges. Our service begins with thorough construct design, considering codon optimization, solubility tags, and cleavage sites. We then screen multiple expression systems to identify the optimal host for high yield and correct folding. Our purification process employs multi-step chromatography (affinity, ion exchange, size exclusion) tailored to achieve purity levels up to 98%. Importantly, every purified enzyme batch undergoes rigorous quality control, including activity assays, mass spectrometry, and purity analysis, guaranteeing that the final product is ready for demanding structural or functional drug discovery applications.

Enzyme Production Workflow

A staged process from gene to high-purity, active enzyme.

Construct Design & Gene Synthesis

Expression Screening & Optimization

Large-Scale Purification

Quality Control & Delivery

Gene Optimization: Codon optimization, addition of purification tags (e.g., His-tag), and protease cleavage sites.

Vector Construction: Cloning the target gene into the selected expression vector (e.g., pET, Baculovirus, Mammalian).

Pilot Expression: Small-scale expression in 2-3 selected host systems to compare yield and solubility.

Process Optimization: Fine-tuning induction conditions (temperature, time, inducer concentration) for maximal yield.

Cell Culture and Lysis: Large-scale culture amplification followed by mechanical or chemical lysis.

Multi-Step Chromatography: Sequential purification using affinity chromatography (e.g., Ni-NTA) followed by SEC or IEX to achieve high purity.

Purity Check: Analyze purity using SDS-PAGE and Densitometry (typically >90% or >95%).
Activity Assay: Confirm functionality using an established or custom kinetic assay (specific activity, Kd).
Final Formulation: Buffer exchange, sterile filtration, aliquoting, snap-freezing, and shipment with a detailed CoA.

Reliability and Purity for Demanding Applications

Guaranteed High Purity

Purity guaranteed up to 98%, ensuring minimal background interference for sensitive HTS and structural studies.

Functional Activity Validation

Every batch is tested for specific activity, confirming the enzyme is correctly folded and biologically functional.

Complex Enzyme Expertise

Proven track record with difficult targets, including multi-subunit complexes, membrane proteins, and PTM-dependent enzymes.

Scalability

Ability to scale from milligrams for characterization to gram-scale production for industrial or clinical supply.

Client Testimonials on Enzyme Expression & Purification

"They successfully expressed and purified a notoriously difficult P450 enzyme in an insect cell system, achieving high purity and unprecedented activity for our screening program."

Dr. Emma Li, Structural Biology Lead

"The low endotoxin grade enzyme was perfect for our cell-based assays, and the team was highly communicative throughout the entire construct optimization phase."

Mr. Omar Hassan, In Vitro Pharmacology Director

"We received grams of our target kinase purified to >98% homogeneity. This reliability has been critical for our late-stage lead optimization."

Ms. Vivianne Dubois, HTS Manager

FAQs about Enzyme Expression and Purification

Still have questions?

Why do you offer multiple expression systems?

Different enzymes require different environments for correct folding and activity. E. coli is fast and cheap but lacks PTMs. Mammalian systems (HEK/CHO) are necessary for complex PTMs, while Insect cells (Baculovirus) are often ideal for large, complex proteins and membrane receptors.

What is the difference between purity and activity?

Purity (measured by SDS-PAGE) refers to the percentage of the target protein relative to total protein. Activity (measured by a functional assay) refers to the biological function (e.g., specific activity in U/mg) of that protein. High purity does not guarantee high activity if the protein is misfolded.

Can you purify membrane proteins?

Yes. We have specialized expertise in expressing and purifying membrane-associated enzymes and receptors, utilizing optimized detergent screening and stabilization protocols to maintain their integrity and activity.

Is Endotoxin removal included in the service?

Endotoxin testing is a selectable QC module. We offer specialized Endotoxin removal steps to ensure the final product meets the necessary safety standards (Low Endotoxin grade) for subsequent cell-based or animal studies.

How much does Metabolic Engineering services cost?

The cost of Metabolic Engineering services depends on the project scope, complexity of the target compound, the host organism chosen, and the required yield optimization. We provide customized quotes after a detailed discussion of your specific research objectives.

Do your engineered strains meet regulatory standards?

We adhere to high quality control standards in all strain construction and optimization processes. While we do not handle final regulatory approval, our detailed documentation and compliance with best laboratory practices ensure your engineered strains are prepared for necessary regulatory filings (e.g., GRAS, FDA).

What to look for when selecting the best gene editing service?

We provide various gene editing services such as CRISPR-sgRNA library generation, stable transformation cell line generation, gene knockout cell line generation, and gene point mutation cell line generation. Users are free to select the type of service that suits their research.

Does gene editing allow customisability?

Yes, we offer very customised gene editing solutions such as AAV vector capsid directed evolution, mRNA vector gene delivery, library creation, promoter evolution and screening, etc.

What is the process for keeping data private and confidential?

We adhere to the data privacy policy completely, and all customer data and experimental data are kept confidential.

Enzyme Expression and Purification Services

Customized Production of High-Quality, Active Recombinant Enzymes

Customizable Enzyme Production Modules

Choose Your Expression Host

Select the optimal host system based on enzyme complexity and PTM requirements:

Select Purification Tags and Services

Select the required tags, cleavage options, and complex purification modes:

Quality Control and Purity Assurance

Select the required QC assays to confirm enzyme function and safety:

Enzyme Production Workflow

Reliability and Purity for Demanding Applications

Client Testimonials on Enzyme Expression & Purification

FAQs about Enzyme Expression and Purification