Enzyme Optimization Services

CD Biosynsis offers comprehensive Enzyme Optimization Services designed to overcome the challenges of stability, activity, and specificity faced by natural enzymes in industrial and biopharmaceutical applications. Utilizing a strategy that combines directed evolution and rational design, we precisely tune various enzyme properties. Our services cover enhancing enzyme stability under extreme conditions (such as high temperatures, organic solvents); altering the enzyme's substrate range and product orientation to improve selectivity and specificity; and designing multi-enzyme cascade reactions for efficient one-pot conversion in complex biosynthesis pathways. We are committed to providing customers with custom enzyme preparations that feature superior performance, meet industrial demands, significantly reduce production costs, and improve product purity.

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Precisely Tuning Industrial Performance Parameters of Enzymes

Native enzymes rarely meet the stringent requirements of industrial biocatalysis directly. For instance, enzymes used in chiral drug synthesis must exhibit extremely high enantioselectivity, while enzymes for high-temperature fermentation require exceptional thermal stability. Our enzyme optimization services focus specifically on these critical performance indicators. By employing rational design guided by structural information and directed evolution supported by high-throughput screening, we can precisely modify the enzyme's active site, flexible regions, and surface charge. Particularly in Enzyme Cascade Design, we solve complex issues such as substrate inhibition, cofactor regeneration, and intermediate channeling in multi-enzyme systems, enabling efficient and sustainable one-pot synthesis of high-value complex products from simple starting materials.

Core Enzyme Optimization and Engineering Projects

Environmental Tolerance Enhancement Catalytic Function Refinement Complex Synthesis Pathway Construction

Improving Enzyme Survival and Longevity

Stability Engineering

Enzyme Stability Engineering Service

Enhances enzyme thermostability, pH stability, and solvent tolerance through rational design methods like increasing disulfide bonds, optimizing surface charge networks, and stiffening flexible loops, combined with directed evolution.

Thermostability Enhancement

For high-temperature reaction environments, we can increase the enzyme's melting temperature (Tm) by 10 degrees Celsius or more through library screening, significantly extending its half-life.

Immobilization Optimization

Optimizing the coupling sites and methods between the enzyme and carrier to improve the operational stability and reusability of immobilized enzymes.

Altering Substrate and Product Orientation

Specificity and Selectivity Engineering

Enzyme Specificity Selectivity Engineering Service

Achieves catalysis of non-natural substrates or alters the enzyme's enantioselectivity (ee value) by remodeling the active site pocket structure, altering steric hindrance, or modifying the electrostatic environment of the substrate channel.

Substrate Scope Alteration

Broadening the enzyme's catalytic range to entirely new substrate families, or conversely, focusing it on specific structures to meet particular biosynthetic needs.

Chirality Control

Optimizing the stereoselectivity of chiral catalysts (such as reductases or lipases) to achieve enantiomeric excess (ee) greater than 99 percent.

Multi-Enzyme System Integration and Pathway Optimization

Enzyme Cascade Design

Enzyme Cascade Design Service

Design and optimize biosynthetic pathways containing two or more enzymes to achieve efficient "one-pot" conversion from substrate to final product, resolving intermediate accumulation issues.

Cofactor Regeneration

Design efficient cofactor (e.g., NAD(P)H) regeneration systems to ensure continuous function of oxidoreductases in the cascade reaction, reducing costs.

Spatial Organization

Utilize protein scaffolds or engineered organelles to spatially organize enzymes in the cascade reaction, enabling product channeling and improving overall conversion yield.

Enzyme Optimization Service Workflow

A customized closed loop from target setting to performance validation.

Performance Analysis and Goal Setting

Design Strategy Selection and Library Construction

High-Throughput Screening and Enrichment

Variant Validation and Delivery

Pain Point Identification: Analyze shortcomings of the current enzyme in terms of activity, stability, or specificity.

Optimization Goal Quantification: Set clear performance metrics, such as a 10 degree Celsius increase in Tm or achieving an ee value of 99.5 percent.

Strategy Determination: Select between rational design (based on structural analysis) or directed evolution (library screening).

Library Construction: Generate high-diversity saturation mutagenesis or DNA Shuffling libraries based on the chosen strategy.

Customized Screening: Develop HTS or display screening methods specifically targeting the optimization goal (e.g., product selectivity or thermal deactivation).

Iterative Optimization: Screen the best variants and use them as parents for subsequent rounds of evolution.

Performance Validation: Conduct detailed kinetic, stability, and specificity validation of the final variants.
Structural Analysis (Optional): Perform structural analysis of the optimized enzyme to explain the molecular mechanism of performance improvement.
Delivery: Final optimized gene sequence, expression vector, and a detailed performance report.

Unique Benefits of Our Enzyme Optimization Services

Rational and Evolutionary Synergy

Combines CADD (Computer-Aided Drug Design) with high-throughput screening for targeted and highly efficient optimization.

Focus on Industrial Metrics

Optimization goals are directly aligned with industrial production needs, such as improving conversion yield, reducing cost, and extending lifespan.

Complex Cascade Capability

Expertise in designing multi-enzyme systems, cofactor regeneration, and intermediate channeling for complex engineering.

Customized Screening Models

Development of bespoke HTS assays for hard-to-quantify metrics like selectivity and specificity.

Client Testimonials on Enzyme Optimization Services

"Through the enzyme stability engineering service, we obtained a lipase with a 10-fold extended half-life at 60 degrees Celsius, significantly improving our biodiesel production process."

Dr. Samuel Zhang, Industrial Biotechnology R&D Manager

"Their engineering of enzyme selectivity and specificity raised the ee value of our chiral drug synthesis key step from 92 percent to 99.8 percent, fully meeting pharmacopoeia standards."

Ms. Janet Li, Pharmaceutical Company Chief Engineer

"The enzyme cascade design perfectly solved the cofactor regeneration issue, integrating a three-step conversion reaction into one-pot, significantly boosting our conversion efficiency and reducing costs."

Prof. Ken Wang, Green Chemistry Project Lead

FAQs about Enzyme Optimization Services

Still have questions?

What factors are primarily addressed in enzyme stability engineering?

Primarily thermal deactivation, pH deactivation, organic solvent tolerance, and deactivation caused by proteolysis. We address these issues through directed evolution and rational design.

How do you evaluate the optimization effect of enantioselectivity (ee value)?

We typically use High-Performance Liquid Chromatography (HPLC) or Gas Chromatography (GC) coupled with a chiral column to conduct high-precision quantitative analysis of the product's enantiomeric excess.

Can enzyme cascade design solve the cofactor consumption problem?

Yes, we typically design an additional cofactor regeneration enzyme (such as formate dehydrogenase) and integrate it into the cascade reaction to achieve in situ, cyclical regeneration of the cofactor, greatly reducing consumption.

How do rational design and directed evolution differ in stability optimization focus?

Rational design relies on the enzyme's three-dimensional structure to improve stability by modifying specific sites (e.g., adding disulfide bonds, optimizing salt bridges). Directed evolution discovers unexpected beneficial mutations through library screening, often more suitable for complex optimizations with unclear mechanisms.

How much does Metabolic Engineering services cost?

The cost of Metabolic Engineering services depends on the project scope, complexity of the target compound, the host organism chosen, and the required yield optimization. We provide customized quotes after a detailed discussion of your specific research objectives.

Do your engineered strains meet regulatory standards?

We adhere to high quality control standards in all strain construction and optimization processes. While we do not handle final regulatory approval, our detailed documentation and compliance with best laboratory practices ensure your engineered strains are prepared for necessary regulatory filings (e.g., GRAS, FDA).

What to look for when selecting the best gene editing service?

We provide various gene editing services such as CRISPR-sgRNA library generation, stable transformation cell line generation, gene knockout cell line generation, and gene point mutation cell line generation. Users are free to select the type of service that suits their research.

Does gene editing allow customisability?

Yes, we offer very customised gene editing solutions such as AAV vector capsid directed evolution, mRNA vector gene delivery, library creation, promoter evolution and screening, etc.

What is the process for keeping data private and confidential?

We adhere to the data privacy policy completely, and all customer data and experimental data are kept confidential.